Application of sequence-based methods in human microbial ecology

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 2.
Figure 2.

Environmental genomic tags for functional analysis of complex microbial communities. Genomic DNA is extracted from the community, sheared, and cloned into a standard sequencing vector. Clones are sequenced and function assigned to individual reads based on highly significant BLAST hits. These reads are then binned by function, and the relative abundance of reads in each functional category can be clustered and displayed graphically so that multiple environments can be compared. The clustering algorithm also computes and displays branch lengths that represent relatedness of individual samples.

This Article

  1. Genome Res. 16: 316-322

Preprint Server