Comparative primate FISH of individual duplicons. Two examples of comparative metaphase FISH experiments for the (A) IGSF3 (dark green) duplicon from 1p13 and the (B) MLL3 duplicon (in yellow) from 7q36 are shown. Extracted metaphases for five primates are shown after hybridization with probes corresponding to the two duplicons: HSA indicates H. sapiens; PTR, P. troglodytes; GGO, G. gorilla; PPY, P. pygmaeus; and MFA, M. fascicularis. Both sets of experiments show multiple signals among humans and the great-apes with a single signal in the Old World monkey macaque. These results are consistent with the phylogenetic and comparative genomic hybridization experiments that suggest a duplication of the ancestral locus <23 Mya. All chromosomal designations are with respect to the human phylogenetic group (McConkey 2004).
