Table 1.
The C. elegans ptc and ptr genes promote molting
|
|
N2 |
rrf-3 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
No.a |
% Mlt |
Stageb |
No.a |
% Mlt |
Stageb |
ESTs |
||||
| control | 204 | 0 | 185 | 0 | |||||||
| ptc-1c | 375 | 0.8 | 200 | 1 | 11 | ||||||
| ptc-3d | 237 | 70.4 | L2-L3 | 200 | 45 | L1-L2 | 16 | ||||
| ptr-1 | 191 | 0 | 252 | 13.1 | L4-A | 0 | |||||
| ptr-2e | 157 | 25 | L4-A | 188 | 32 | L4-A | 13 | ||||
| ptr-3 | 170 | 6 | L4-A | 225 | 8 | L4-A | 3 | ||||
| ptr-4d | 235 | 94 | L4-A | 320 | 100 | L3-L4 | 16 | ||||
| ptr-5 | 255 | 0 | 310 | 3.2 | L4-A | 12 | |||||
| ptr-6 | 276 | 0 | 270 | 11.1 | L4-A | 3 | |||||
| ptr-7 | 253 | 0 | 340 | 0 | 1 | ||||||
| ptr-8 | 234 | 0 | 167 | 4.8 | L4-A | 9 | |||||
| ptr-9 | 287 | 0 | 266 | 0 | 0 | ||||||
| ptr-10 | 236 | 2.5 | L4-A | 253 | 6.3 | L4-A | 2 | ||||
| ptr-11 | 237 | 0 | 265 | 0 | 5 | ||||||
| ptr-12 | 240 | 0 | 198 | 4.5 | L4-A | 2 | |||||
| ptr-13 | 286 | 0 | 222 | 5.4 | L4-A | 0 | |||||
| ptr-14 | 258 | 0 | 360 | 6.4 | L4-A | 8 | |||||
| ptr-15 | 270 | 0 | 281 | 0 | 0 | ||||||
| ptr-16 | 207 | 0 | 333 | 31.2 | L2-L3 | 0 | |||||
| ptr-17 | 222 | 0 | 302 | 0 | 1 | ||||||
| ptr-18 | 292 | 18 | L4-A | 262 | 91.6 | L3-L4 | 5 | ||||
| ptr-19 | 239 | 0 | 282 | 0 | 0 | ||||||
| ptr-20 | 245 | 4 | L4-A | 312 | 11.5 | L4-A | 4 | ||||
| ptr-21 | 249 | 0 | 198 | 9.6 | L4-A | 0 | |||||
| ptr-22 | 240 | 0 | 200 | 8 | L3-L4 | 0 | |||||
| ptr-23 | 276 | 81.5 | L4-A | 330 | 94 | L4-A | 19 | ||||
| ptr-24
|
153
|
0
|
|
298
|
0
|
|
15
|
||||
-
↵a No. represents results from at least three independent experiments
-
↵b The earliest stage showing molting defects
-
↵c RNAi was performed on L1 stage animals to bypass the embryonic requirement for ptc-1 activity (Kuwabara et al. 2000)
-
↵d 100% of ptc-3(RNAi) and ptr-4(RNAi) animals subsequently die
-
↵e 90% of ptr-2(RNAi) animals display embryonic lethality (Emb); hence, only animals surviving this lethality were scored
