Analysis of Sequence Variations in Several Human Genes Using Phosphoramidite Bond DNA Fragmentation and Chip-Based MALDI-TOF

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Figure 2Figure 2
Figure 2

Fragmentation analysis of an APOB target by using an optimized cleavage protocol. (A) Sequence of a 265-bp APOB amplicon and the list of fragments resulting from a P-N dT-specific cleavage of an extension product. Locations of PCR and extension primers are labeled. Potential cleavage sites (dT) are indicated in bold. (B) Experimentally obtained fragmentation mass spectrum of a 265-bp product. Only measured peak m/z values are shown.

This Article

  1. Genome Res. 14: 134-141

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