

Fragmentation analysis of an APOB target by using an optimized cleavage protocol. (A) Sequence of a 265-bp APOB amplicon and the list of fragments resulting from a P-N dT-specific cleavage of an extension product. Locations of PCR and extension primers are labeled. Potential cleavage sites (dT) are indicated in bold. (B) Experimentally obtained fragmentation mass spectrum of a 265-bp product. Only measured peak m/z values are shown.











