Figure 9
Mutations in okra do not affect eccDNA. DNA from adult flies carrying the following genotypes was separated on 2D gel: (A,D) okrA17-11/okrAG; (B) okrA17-11/Df(2L)JS17; (C) okrA17-11/CyO. The blots in panels A—C were hybridized with total genomic DNA probe; in panel D, a 5S rDNA probe was used. No change in the relative amount of eccDNA was observed in the mutant genotypes compared with the heterozygous one. The circular 5S rDNA multimer organization in the mutants is similar to that observed in the wild type (cf. panel D to Fig. 4).
