Expression of Reporter Constructs in Primary Transgenic Mouse Embryos and Transgenic Mouse Line
| Enhancer element | Primary (P) | Lines (L) | Ventral forebrain | Frontonasal prominence | Apical ectodermal ridge | Visceral arches | Ectopic expression |
| MI12a | N.D. | 7 | 0L | 0L | 1L | 6L | 2L |
| ZI12a | N.D. | 2 | 0L | 0L | 0L | 1L | 0L |
| MI12b | 3 | 5 | 3P, 5L | 3P, 4L | 3P, 5L | 1P, 0L | 2P, 1L |
| ZI12b | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
| MI56i | 13 | 4 | 13P, 3L | 2P, 1L | 1P, 0L | 7P, 2L | N.D. |
| ZI56i | 12 | N.D. | 12P | 0P | 0P | 3P | N.D. |
| MI56ii | 3 | 2 | 3P,2L | 1P, 0L | 2P, 1L | 0P, 0L | 1P, 0L |
| ZI56ii | 10 | 2 | 1P, 0L | 1P, 1L | 1P, 0L | 0P, 2L | N.D. |
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All constructs were made by inserting the enhancer fragments into the p1229/1230 plasmids that contain the LacZ reporter gene under the control of a β-globin minimal promoter. M, mouse, Z, zebrafish.
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↵Weak expression in the second arch.
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↵Both primaries and lines showed variable expression patterns in the two domains of the forebrain; thus, 2P showed a weaker expression pattern in the telencephalon (domain II) compared to the diencephalon (domain I), whereas, 2L showed expression only in the diencephalon (domain I), and 1P (shown in Fig. 6B) showed an equally strong expression in both domains (I, II).
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↵From Zerucha et al. 2000.
