Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection

  1. Rita Sousa-Nunes1,10,
  2. Amer Ahmed Rana1,10,7,
  3. Ross Kettleborough1,10,
  4. Joshua M. Brickman1,8,
  5. Melanie Clements1,
  6. Alistair Forrest2,
  7. Sean Grimmond2,
  8. Philip Avner3,
  9. James C. Smith4,11,
  10. Sally L. Dunwoodie1,5,6,11, and
  11. Rosa S.P. Beddington1,9
  1. 1 Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom
  2. 2 Institute of Molecular Bioscience, University of Queensland, 4072 Australia
  3. 3 Unité Génétique Moléculaire Murine, Institut Pasteur, 75015 Paris, France
  4. 4 Wellcome Trust/Cancer Research UK Institute and Department of Zoology, University of Cambridge, Cambridge CB2 1QR, United Kingdom
  5. 5 Developmental Biology Program, Victor Chang Cardiac Research Institute, Darlinghurst, 2010, Australia
  6. 6 Department of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, NSW 2033, Australia

Abstract

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node–tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

Footnotes

  • [Supplemental material is available online at www.genome.org.]

  • Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.1362303. Article published online before print in November 2003.

  • ↵10 These authors contributed equally to the work described.

  • ↵7 Present address: Wellcome Trust/Cancer Research UK Institute, Cambridge CB2 1QR, UK

  • ↵8 Present address: Institute for Stem Cell Research, The University of Edinburgh, Edinburgh EH9 3JQ, UK.

  • ↵11 Corresponding authors. E-MAIL jim{at}welc.cam.ac.uk; FAX 44-1223-33413. E-MAIL s.dunwoodie{at}victorchang.unsw.edu.au; FAX: 61-02-9295-8501.

  • ↵9 Deceased May 18, 2001.

    • Accepted September 18, 2003.
    • Received March 24, 2003.
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  1. Published in Advance January 1, 2003, doi: 10.1101/gr.1362303 Genome Res. 13: 2609-2620 Cold Spring Harbor Laboratory Press

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