Sequence alignment of the Ccc2a amino acid sequence from yeast S. cerevisiae with the heavy metal-binding domains located in the present research. At the top, amino acid numbering is reported including gaps (first line) and according to the sequence of Ccc2a (second line). The two metal-binding cysteines are shaded in blue. Key negative (Glu and Asp) and positive (Arg and Lys) residues are indicated in red and blue, respectively. Positions where hydrophobic residues are conserved are highlighted in green. The two boxes include eukaryotic (green) and bacterial (blue) sequences. The secondary structure elements reported above the alignment are referred to Ccc2a. In column a we report sequence identity to Ccc2a; in column b the length of interdomain linkers for multidomain proteins. 1 gi‖6320475 S. cerevisiae,1st domain (Ccc2a); 2 S. cerevisiae, 2nd domain (Ccc2b); 3 gi‖7492267 Schizosaccharomyces pombe;4 gi‖8745541 Candida albicans, 1st domain; 5 C. albicans, 2nd domain; 6 gi‖6633848 A. thaliana I, 1st domain; 7 A. thaliana I, 2nd domain; 8 gi‖12229667 A. thaliana II, 1st domain;9 A. thaliana II, 2nd domain; 10 gi‖1351993 human Menkes protein, 1st domain (MNK1); 11 human Menkes protein, 2nd domain (MNK2); 12 human Menkes protein, 3rd domain (MNK3); 13 human Menkes protein, 4th domain (MNK4); 14human Menkes protein, 5th domain (MNK5); 15 human Menkes protein, 6th domain (MNK6); 16 gi‖14758520 human Wilson protein, 1st domain; 17 human Wilson protein, 2nd domain;18 human Wilson protein, 3rd domain; 19 human Wilson protein, 4th domain; 20 human Wilson protein, 5th domain;21 human Wilson protein, 6th domain; 22 gi‖2739170O. aries, 1st domain; 23 O. aries, 2nd domain; 24 O. aries, 3rd domain; 25 O. aries, 4th domain; 26 O. aries, 5th domain;27 O. aries, 6th domain; 28 gi‖2440287M. musculus I, 1st domain; 29 M. musculus I, 2nd domain; 30 M. musculus I, 3rd domain; 31 M. musculus I, 4th domain; 32 M. musculus I, 5th domain; 33 M. musculus I, 6th domain; 34gi‖12229577 M. musculus II, 1st domain; 35 M. musculus II, 2nd domain; 36 M. musculus II, 3rd domain; 37 M. musculus II, 4th domain; 38 M. musculus II, 5th domain; 39 gi‖1351992Cricetulus griseus, 1st domain; 40 C. griseus, 2nd domain; 41 C. griseus, 3rd domain;42 C. griseus, 4th domain; 43 C. griseus, 5th domain; 44 C. griseus, 6th domain;45 gi‖12229551 R. norvegicus I, 1st domain;46 R. norvegicus I, 2nd domain; 47 R. norvegicus I, 3rd domain; 48 R. norvegicus I, 4th domain; 49 R. norvegicus I, 5th domain; 50 R. norvegicus I, 6th domain; 51 gi‖6006293 R. norvegicus II, 1st domain; 52 R. norvegicus II, 2nd domain; 53 R. norvegicus II, 3rd domain;54 R. norvegicus II, 4th domain; 55 R. norvegicus II, 5th domain; 56 gi‖7428301 C. elegans, 1st domain; 57 C. elegans, 2nd domain;58 C. elegans, 3rd domain; 59 gi‖7292707D. melanogaster, 1st domain; 60 D. melanogaster, 2nd domain; 61 D. melanogaster, 3rd domain; 62 D. melanogaster, 4th domain; 63gi‖10122140 Cryptosporidium parvum; 64 gi‖7531047B. subtilis, 1st domain (CopAa); 65 B. subtilis, 2nd domain (CopAb); 66 gi‖15283450 Aquifex aeolicus; 67 gi‖1354935 Escherichia coli, 1st domain; 68 E. coli, 2nd domain; 69gi‖2493017 Haemophilus influenzae; 70 gi‖8894835Streptomyces coelicolor I; 71 gi‖6689151 S. coelicolor II; 72 gi‖6714779 S. coelicolor III;73 gi‖2493001 Synechocystis I; 74gi‖7428304 Synechocystis II; 75 gi‖11267419Campylobacter jejuni; 76 gi‖7471220 Deinococcus radiodurans, 1st domain; 77 D. radiodurans, 2nd domain; 78 gi‖416665 E. hirae (CopA); 79gi‖2493003 H. pylori, strain 26695; 80 gi‖3121870H. felis; 81 gi‖13432119 Mycobacterium leprae I; 82 gi‖13637888 M. leprae II; 83gi‖1706183 Mycobacterium tuberculosis I; 84gi‖1706184 M. tuberculosis II; 85 gi‖11267401Neisseria meningitidis, serogroup A strain Z2491; 86gi‖11267405 N. meningitidis, serogroup B strain MC58;87 gi‖1353678 Proteus mirabilis, 1st domain;88 P. mirabilis, 2nd domain; 89 gi‖7531049Sinorhizobium meliloti, 1st domain; 90 S. meliloti, 2nd domain; 91 gi‖584792 Synechococcus I; 92 gi‖584820 Synechococcus II; 93gi‖7436397 Thermotoga maritima; 94 gi‖14601418Aeropyrum pernix; 95 gi‖11498084 Archaeoglobus fulgidus; 96 gi‖7428308 Methanobacterium thermoautotrophicum, 1st domain; 97 M. thermoautotrophicum, 2nd domain; 98 gi‖8388793Pseudomonas syringae, 1st domain; 99 P. syringae, 2nd domain; 100 gi‖9965435 S. mutans; 101 gi‖13622770 S. pyogenes;102 gi‖10173170 B. halodurans, 1st domain;103 B. halodurans, 2nd domain; 104gi‖11351437 P. aeruginosa, 1st domain; 105 P. aeruginosa, 2nd domain; 106 gi‖12723759 L. lactis; 107 gi‖10580282 Halobacterium sp., strain NRC-1, 1st domain; 108 Halobacterium sp., strain NRC-1, 2nd domain; 109 gi‖12722323 P. multocida; 110 gi‖120199 Sinorhizobium meliloti (fixI); 111 gi‖3122077 Rhizobium leguminosarum; 112 gi‖3122094 Bradyrhizobium japonicum; 113 gi‖13475529 Mesorhizobium loti;114 gi‖2338745 Rhodobacter capsulatus;115 gi‖7271788 Rhodobacter sphaeroides; 116gi‖13422766 Caulobacter crescentus.
