Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition

Genomic organization, polymorphisms, and haplotypes found in the bovineDGAT1 gene. Leader and trailer sequences are shown in green, coding sequences in red, and intronic sequences in gray. The positions of the four identified polymorphisms are marked by yellow lines on the gene, and detailed in the underlying boxes including the corresponding sequence traces. The four DGAT1 haplotypes which were found in the Dutch and New Zealand Holstein-Friesian population are shown and referred to as “sH^Q-D ”, “sH^Q-NZ ”, and “sH^Q-III ” for the fat-increasing haplotypes and “sh^q “ for the fat-decreasing haplotype.