(See figure on page 224.) Generation of a BAC contig spanning the BULGE13-BULGE09 interval. The most likely position of the QTL (Farnir et al. 2002) is shown as a red bar on the FISH-anchored linkage map of proximal BTA14q. The BACs composing the contigs spanning the BULGE13-BULGE09 interval are shown as a series of black horizontal lines. The dots on each BAC indicate their individual STS content: black dots correspond to STSs derived from BAC ends, green dots to microsatellite markers, and red dots to gene-specific comparative anchored tagged sequences (CATS;Lyons et al. 1997). Black arrowheads mark the BACs from which the respective BAC and STS were derived. The length of the lines do not reflect the actual insert size of the corresponding BACs. The BAC contig is aligned with the orthologous human HSA8q24.3 genomic “golden path” sequence (Lander et al. 2001) represented according to the Ensembl Human Genome Server (http://www.ensembl.org/): individual sequence contigs are shown in alternating dark and light blue; a horizontal line indicates a gap in the sequence assembly; genetic markers are indicated in green under the contig map; and green, red, and black boxes represent “curated,” “predicted known,” and “predicted novel” genes, respectively.
