Table 6.
Plasmids, Strains, and Primers
| Plasmids | Relevant properties | Reference |
| pMutin2 | pBR322-based integration vector for B. subtilis; containing a multiple cloning site downstream of the Pspac promoter, and a promoterless lacZ-gene preceded by the RBS of thespoVG gene; 8.6 kb; APr; Emr | Vagner et al. 1998 |
| Strains | ||
| E. coli | ||
| MC1061 | F−; araD139; Δ (ara-leu)7696; Δ (lac)X74; galU; galK; hsdR2; mcrA; mcrB1; rspL | |
| B. subtilis | ||
| 168 | trpC2 | Kunst et al. 1997 |
| ΔS | like 168; sipS; Kmr | This paper |
| ΔT | like 168; sipT; Cmr | This paper |
| ΔU | like 168; sipU; Kmr | This paper |
| ΔV | like 168; sipV; Spr | This paper |
| ΔW | like 168; sipW; Tcr | This paper |
| ΔTV | like 168; sipT; Cmr;sipV; Spr | This paper |
| ΔTUV | like 168; sipT; Cmr; sipU; Kmr;sipV; Spr | This paper |
| ΔTUVW | like 168; sipT; Cmr; sipU; Kmr;sipV; Spr; sipW; Tcr | This paper |
| ΔlspA | like 168; lspA::pMutin2;lsp-lacZ; Emr | This paper |
| Δlgt | like 168; lgt::pMjtin2; lgt-lacZ; Emr: also known as LUH104 | Bengston et al. 1999 |
| QB136 | trpC2, leuA8, degU32(hy) | Kunst et al. 1974 |
| Primers | 5′–3′ | |
| LytDvor | AGGCACAACAACGACCTAC | This paper |
| LytDT7rev | CTAATACGACTCACTATAGGGAGATCTGCTGTTGATCCAGCTG | This paper |
| XepAvor | GCATACAGTCAGCTCGGG | This paper |
| XepAT7rev | CTAATACGACTCACTATAGGGAGAAATGTACTGGAGGCAGTGG | This paper |
| XlyAvor | GAAAACGGCTGGCATGCG | This paper |
| XlyAT7rev | CTAATACGACTCACTATAGGGAGATGCGTCAGCTGTTTTCGG | This paper |
| YfiYvor | TAATGAAGGAACAGAGGCG | This paper |
| YfiYrev | CTAATACGACTCACTATAGGGAGATTTCCATAGTGAACTGCTTG | This paper |
| OppAvor | AATGATTCAGTATCAGGCGG | This paper |
| OppArev | CTAATACGACTCACTATAGGGAGATACTGCTTGAGCGATTTTCG | This paper |
| MntAvor | TTGCGACCTTTGCTTTAACG | This paper |
| MntArev | CTAATACGACTCACTATAGGGAGATCTTTTGTGCCCTTTTCACC | This paper |
| YvcEvor | AGCAATCTGAGGTTGCTTCC | This paper |
| YvcET7rev | CTAATACGACTCACTATAGGGAGACTTGAACCAACGCTGATCGC | This paper |
| YxeBvor | GTCACTGATTTTTATGCCGG | This paper |
| YxeBT7rev | CTAATACGACTCACTATAGGGAGAGTCGTACGGGTAGAACGAGT | This paper |
