Amino Acid Residues around (Putative) SPase I Cleavage Sites
| position −3 | position −2 | position −1 | position +1 | ||||
| residue | [%] | residue | [%] | residue | [%] | residue | [%] |
| A | 68 (60) | S | 22 (18) | A | 100 (85) | A | 43 (27) |
| V | 20 (18) | K | 12 (10) | Q | 14 (10) | ||
| T | 4 (6) | E | 12 (8) | E | 10 (1) | ||
| I | 2 (6) | H | 9 (6) | K | 6 (10) | ||
| S | 2 (5) | Y | 9 (2) | S | 6 (8) | ||
| G | 2 (5) | Q | 6 (7) | V | 6 (5) | ||
| W | 2 (<1) | G | 6 (4) | F | 4 (5) | ||
| F | 4 (11) | N | 2 (4) | ||||
| L | 4 (8) | L | 2 (4) | ||||
| A | 4 (7) | D | 2 (4) | ||||
| D | 4 (5) | T | 2 (2) | ||||
| N | 4 (4) | ||||||
| W | 2 (1) | ||||||
| P | 2 (<1) | ||||||
| L,F,Q | G,S,V,L, | I,H,Y,G, | |||||
| M,K,Y | 0 (4) | I,V,T,R,M | 0 (16) | E,I,N | 0 (15) | R,W,M | 0 (14) |
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The frequency of a particular amino acid at the indicated positions around SPase I cleavage sites in signal peptides of identified proteins is given as the percentage of the total number (50) of identified (pre-)proteins with a SPase I cleavage site. Putative SPase I cleavage sites were derived from the 39 previously-predicted signal peptides, the two unpredicted signal peptides of YweA and YxkZ, the signal peptides of the four proteins with a cell wall-binding domain, the signal peptide of the previously predicted lipoprotein YrpD, and the “signal peptides” of the four putative membrane proteins YfkN, YhcR, YfnI and XynD (see Fig. 2 and Table 1). The numbers in parentheses represent previously-reported, genome-based, predicted frequencies (Tjalsma et al. 2000). Residues that were predicted previously to reside at one of the indicated positions and that are not represented in the identified (pre-)proteins are listed at the bottom of each column.
