The extracellular proteome of B. subtilis degU32(hy). Cells ofB. subtilis degU32(hy) were grown in L-broth and proteins in the growth medium were harvested 1 h after entry into the stationary phase. After precipitation with TCA, the extracellular proteins were separated by 2D PAGE as described in the Methods section. The proteins identified by mass spectrometry are indicated. Extracellular proteins of which the synthesis is induced in the degU32(hy) mutant are boxed, and those of which the synthesis is reduced are printed in bold (see also the listing in Table 2). This gel is available in the Sub2D proteome database (http://microbio2.biologie.uni-greifswald.de:8880/).
