A Novel Method of Gene Transcript Profiling in Airway Biopsy Homogenates Reveals Increased Expression of a Na+-K+-Cl− Cotransporter (NKCC1) in Asthmatic Subjects

Table 1.

Two-Step RT-PCR for Quantification of Gene Expression in Varying Numbers of CD4+ Cells from Bronchoalveolar Lavage

Number of CD4+cells Ct dCt
IL8 IL13 GAPDH IL8/GAPDH IL13/GAPDH
  4 16.71 19.99 20.18 3.47 0.19
 16 14.32 17.91 18.33 4.01 0.42
 64 13.18 16.83 17.47 4.29 0.64
128 12.05 16.07 16.72 4.67 0.65

This Article

  1. Published in Advance August 16, 2001, doi: 10.1101/gr.191301 Genome Res. 11: 1473-1483

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