Figure 3.
Amplification of pUC19 from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with EcoRI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.
