Sequence-Based Design of Single-Copy Genomic DNA Probes for Fluorescence In Situ Hybridization

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Figure 2.
Figure 2.

Long PCR amplification. Amplification products are separated by gel electrophoresis in 1% low-melt temperature agarose in modified TAE (40 mM Tris-acetate, pH 8.0, 0.1 mM Na2EDTA). Lanes 1and 2 show the 2290-bp and 3544-bp products from theMAGEL2 locus; lanes 3– 6 contain, respectively, the 2848-bp, 3344-bp, 3691-bp, and 5170-bp products from the HIRA locus; lanes 7 and 8 are the 4724-bp and 4823-bp products, respectively, from the CDC2L1 locus.

This Article

  1. Genome Res. 11: 1086-1094

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