Homogeneous Assays for Single-Nucleotide Polymorphism Typing Using AlphaScreen

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Figure 4.
Figure 4.

AlphaScreen ASH genotyping assay of SNP WIAF-896. (A) AlphaScreen signal obtained from 21 CEPH genomic DNA samples after allele-specific hybridization of the detection probes at 54.5°C. (solid bars) Samples amplified with the A-specific PCR primer; (white bars) samples amplified with the C-specific primer. (Ctrl) No DNA sample. (B) Log plot of the ratio of the AlphaScreen signal obtained for the two alleles (A/C). Each point represents a single CEPH individual. (C) Titration of genomic DNA for the WIAF-896 AlphaScreen ASH assay by using an A/C heterozygote sample.

This Article

  1. Genome Res. 11: 600-608

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