A Clone-Array Pooled Shotgun Strategy for Sequencing Large Genomes

Table 1.

Computational Requirements for Assembly of a 3-Gb Genome

CBC (22,000 BACs) CAPSS (148 × 148 BACs) CAPSS (60 × 60 BACs, six arrays) WGS (10X coverage)
Number of reads/Assembly 3000 203,000 83,300 6.0 × 107
Computer load units/Assembly 1.0 4.5 × 103 7.6 × 102 4.0 × 108
Total number of assemblies 2.2 × 104 296 720 1.0
Total load/Genome 2.2 × 104 1.3 × 106 5.5 × 105 4.0 × 108
Approx. hardware unit cost  (∼$1000's) < 20 < 20 < 20 80,000
Estimated total hardware cost  ($1000's) 100 100 100 80,000

This Article

  1. Genome Res. 11: 1619-1623

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