Figure 3.
Design and specificity of variant-specific oligonucleotide probes. (A) The sequences of all variant-specific oligoprobes, including 15VAR1.4 and 15VAR2.4, are compared to the total and the chromosome 15-specific alphoid consensuses, as well as to clones E2 and F1. The variant position 529 is shown in bold. (B) The variant-specific probes in a Southern blot assay against clones E2 and F1. At low stringency, there is extensive cross hybridization. At high stringency, the probes are specific for the sequences from which they are designed.
