Figure 2.
Sequence variation within chromosome 15-specific alpha satellite DNA. (A) Sequences of cell lines A9 + 15 and GAR1. Total PCR products, generated with primer pair 15SAT7/8, were sequenced using primer 15SAT7. A9 + 15 shows bands in the T and C lanes at position 529, while GAR1 shows a single band in the T lane. (B) Sequences of individual alpha satellite DNA clones. PCR products from both cell lines were cloned and sequenced individually. Clones from A9 + 15 have a T or a C at position 529, while clones from GAR1 only have a T.
