Alpha Satellite DNA Variant-Specific Oligoprobes Differing by a Single Base Can Distinguish Chromosome 15 Homologs

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Figure 1.Figure 1.Figure 1.
Figure 1.

Genomic organization of pTRA-20-specific alpha satellite DNA. (A) A schematic of the 1.8-kb higher-order repeat of chromosome 15 along with the positions of the PCR primers (not drawn to scale). The primers were designed using pTRA-20 sequence. The location of the additional 700 bp is indicated below the 1.8-kb fragment. (B) PCR of monochromosomal somatic cell hybrids A9 + 15 (lanes 1 and 3) and GAR-1 (lanes 2 and4) with primer pairs 15SAT7/8 (lanes 1 and2) and 15SAT9/10 (lanes 3 and 4). 15SAT7/8 amplified a band of the predicted size, 986 bp. 15SAT9/10 amplified a band of 464 bp, indicating that the additional 700 bp is not adjacent to the 1.8-kb pTRA-20 sequence. (C) Southern blots of A9 + 15 and GAR-1 genomic DNA, digested with AccI (A), EcoRI (I), EcoRV (V), HincII (Hc), HindIII (Hd), and StuI (St). The top arrow marks 2.5 kb and the bottom arrow marks 1.8 kb. Both cell lines contain pTRA-20-specific higher-order repeats of 2.5 kb (EcoRV) and 1.8 kb (EcoRI).

This Article

  1. Genome Res. 10: 1342-1350

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