
Location of the primers used for PCR amplifications. Primers (arrows) located in exon 1 and exon 2 were used to amplify the entire intron 1 of DRB genes. The cloned products were typed for the presence of Alu50, Alu62, and Alu63 with combinations of the primers Alu50-I–Alu50-VII and AluSc-I–AluSc-VI. The sequences of the primers are given in Table 1. (E) Exon, the shaded boxes represent Alu elements.











