Table 5.

Oligonucleotide Sequences for the Secondary Invader Reaction

Oligonucleotide	Sequence	3′ modification	5′ label	Internal label (Z)
FRET probe 1	CAACZGCTTCCTCCG	dmf-dG	fluorescein	Cy3
FRET probe 2	TAACZGCTTCCTCCG	dmf-dG	fluorescein	Cy3
Secondary target 1	CGGAGGAAGCAGTTGGTGCGCCTCG^U^U^A^A^*	phosphate
Secondary target 2	CGGAGGAAGCAGTTGTCCGCGAAG^A^U^G^	amino mod C7
Secondary target 3	CGGAGGAAGCAGTTAGTGCGCCTCG^U^U^A^A^*	amino mod C7
Secondary target 4	CGGAGGAAGCAGTTATCCGCGAAG^A^U^G^	amino mod C7
Secondary target 5	CGGAGGAAGCAGTTATCCGCGAAGAU^G^G^U^	amino mod C7
Primary arm 1	AACGCGGCGCAC
Primary arm 2	TTAACGCGGCGCAC
Primary arm 3	CATCTTCGCGGA
Primary arm 4	ACCATCTTCGCGGA

Evaluation of Single Nucleotide Polymorphism Typing with Invader on PCR Amplicons and Its Automation