Predicted structures of IMPACT homologs. (A) Multiple alignment of IMPACT homologs. Identical residues are expressed as white letters in black, and similar residues are shaded. Aligned proteins are Homo sapiens IMPACT (accession no.AB026264), Mus musculus Impact (accession no. D87973),Xenopus laevis Ximpact (accession no. AB020319), and hypotheical proteins including Caenorhabditis elegans Y52B11A.2 (accession no. CAA21718), Schizosaccharomyces pombe SPAC27E2.02 (accession no. CAB11676), S. pombe SPBC14C8.09C (accession no.CAA18427), Saccharomyces cerevisiae YCR059c (accession no.CAA42287.1), S. cerevisiae YDL177c (accession no. Z74225),Arabidopsis thaliana F20D10.210 (accession no. CAB37549.1),Escherichia coli YIGZ (accession no. AAC76851), Bacillus subtilis YVYE/YVHK (accession no. CAB15568/AAC44936), andThermus aquaticus YPOL (accession no. P32438). (B) Schematic presentation of members of UPF0029 hypothetical protein family (PROSITE PS00910). The region B is the core region that is highly conserved among human, mouse, yeast, and various bacteria. They all contain a characteristic signature G-x(2)-[LIMV](2)-x(2)-[LIMV]-x(4)-[LIMV]-x(5)-[LIMV](2)-x-R-[FYW](2)-G-G-x(2)-[LIMV]-G (PROSITE PDOC00707) indicated by asterisks (*). The regions A and C are found only in eukaryotes except for one from A. thaliana, which is rather classified as a prokaryotic type. The number in each box is the identity expressed as a percentage of the corresponding region of the putative IMPACT product. The number in each parentheses indicates the similarity.
