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ARTICLE

Use of a sensitive fluorescent intercalating dye to detect PCR products of low copy number and high molecular weight.

L D Ohler
M Zollo
E S Mansfield
E A Rose

First Published October 1, 1993

ARTICLE

Dealing with contamination: enzymatic control of carryover contamination in PCR.

J L Hartley
A Rashtchian

First Published October 1, 1993

ARTICLE

Degradation of DNA during the denaturation step of PCR.

A Douglas
B Atchison

First Published October 1, 1993

ARTICLE

Modified ASRA facilitates the characterization of activating point mutations in tumors, in which cancer cells constitute only a minor part of the investigated tissue.

T Papp
M Jafari
U Diener
N Dudley
D Schiffmann

First Published October 1, 1993

ARTICLE

Determination of 5' ends of specific mRNAs by DNA ligase-dependent amplification.

W M Bertling
F Beier
E Reichenberger

First Published October 1, 1993

Manual Supplement

Handling Reagents tn the PCR Laboratory

Elizabeth A. Dragon

First Published October 1, 1993

ARTICLE

A PCR-based method for the analysis of human CD44 splice products.

D H van Weering
P D Baas
J L Bos

First Published October 1, 1993

ARTICLES

Selective RNA amplification: a novel method using dUMP-containing primers and uracil DNA glycosylase.

Buchman G W
Schuster D M
Rashtchian A

First Published August 1, 1993

ARTICLES

Detection of exceedingly low levels of HIV proviral DNA in multimillion peripheral blood mononuclear cells by PCR.

P G Babu
B D Rawal
H Khayam-Bashi
G N Vyas

First Published August 1, 1993

ARTICLES

A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses.

W Y Cheung
N Hubert
B S Landry

First Published August 1, 1993

ARTICLES

Simultaneous detection of DNA and RNA by differential polymerase chain reaction (DIFF-PCR).

P Imboden
T Burkart
K Schopfer

First Published August 1, 1993

ARTICLES

Room-temperature-stable PCR reagents.

R Ramanujam
J Koelbl
E Ting
J Jolly
B Burdick

First Published August 1, 1993

ARTICLES

A method for accurate amplification of polymorphic CA-repeat sequences.

S J Odelberg
R White

First Published August 1, 1993

ARTICLES

A simple procedure for enhancing PCR specificity.

F Weighardt
G Biamonti
S Riva

First Published August 1, 1993

ARTICLES

Modified tissue pulverization technique and evaluation of dihydrofolate reductase amplification as a pan-tissue RT PCR control.

P E Tam
A M Schmidt
R P Messner

First Published August 1, 1993

ARTICLES

Detection of unknown gene mutations by multiplex single-strand conformation polymorphism (MSSCP).

F A Saad
L Vitiello
S Oliviero
M L Mostacciuolo
G A Danieli

First Published August 1, 1993

ARTICLES

Automated DNA profiling employing multiplex amplification of short tandem repeat loci.

C P Kimpton
P Gill
A Walton
A Urquhart
E S Millican
M Adams

First Published August 1, 1993

ARTICLES

Fluorescence-based RT PCR analysis: determination of the ratio of soluble to membrane-bound forms of Fc gamma RIIA transcripts in hematopoietic cell lines.

M A Keller
D L Cassel
E F Rappaport
S E McKenzie
E Schwartz
S Surrey

First Published August 1, 1993

ARTICLES

Eliminating mitochondrial DNA competition for nuclear DNA primers.

S Zullo
J L Kennedy
J Gelernter
M H Polymeropoulos
G Tallini
A J Pakstis
M B Shapiro
C R Merril
K K Kidd

First Published August 1, 1993

ARTICLES

Characterization of the extreme 5' ends of RNA molecules by RNA ligation-PCR.

R Sallie

First Published August 1, 1993

ARTICLES

Rapid PCR construction of a gene containing Lym-1 antibody variable regions.

X B Shi
P H Gumerlock
A A Wellman
C F Meares
G L DeNardo
F J Meyers
S J DeNardo

First Published August 1, 1993

ARTICLES

Stimulatory effect of potassium glutamate in PCR.

A Knorr
R T Turner
M E Bolander
G Sarkar

First Published August 1, 1993

ARTICLES

Quantitation of beta-actin-specific mRNA transcripts using xeno-competitive PCR.

R M du Breuil
J M Patel
B V Mendelow

First Published August 1, 1993

REVIEW

Empirical aspects of strand displacement amplification.

G T Walker

First Published August 1, 1993

ARTICLES

Interference of PCR amplification by the polyamines, spermine and spermidine.

H Ahokas
M J Erkkilä

First Published August 1, 1993

ARTICLE

Importance of different variables for enhancing in situ detection of PCR-amplified DNA.

G J Nuovo
F Gallery
R Hom
P MacConnell
W Bloch

First Published May 1, 1993

ARTICLE

A rapid, semiautomated method for apolipoprotein E genotyping.

C Guo
P Marynen
J J Cassiman

First Published May 1, 1993

ARTICLE

Detection of bovine leukemia virus proviral DNA in individual cells.

M L Mirsky
Y Da
H A Lewin

First Published May 1, 1993

ARTICLE

A PCR-derived library of random point mutations within the V3 region of simian immunodeficiency virus.

F Kirchhoff
R C Desrosiers

First Published May 1, 1993

ARTICLE

High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity.

F C Lawyer
S Stoffel
R K Saiki
S Y Chang
P A Landre
R D Abramson
D H Gelfand

First Published May 1, 1993

ARTICLE

Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories.

G A Penner
A Bush
R Wise
W Kim
L Domier
K Kasha
... [+1 authors] ...,
A Laroche
G Scoles
S J Molnar
G Fedak

First Published May 1, 1993

ARTICLE

PCR-based genetic analysis of DNA from autopsied prostate tissue.

J A Macoska
P D Benson
L N Turkeri
G P Haas
W Sakr

First Published May 1, 1993

ARTICLE

Determination of allele frequencies at loci with length polymorphism by quantitative analysis of DNA amplified from pooled samples.

P Pacek
A Sajantila
A C Syvänen

First Published May 1, 1993

ARTICLE

A rapid and simple method for reversing the inhibitory effect of heparin on PCR for HLA class II typing.

F Poli
R Cattaneo
L Crespiatico
A Nocco
G Sirchia

First Published May 1, 1993

ARTICLE

Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations.

A Takahashi-Fujii
Y Ishino
A Shimada
I Kato

First Published May 1, 1993

ARTICLE

Use of stock solutions to simplify mRNA quantitation by reverse transcription--PCR assays.

D Chen
V L Magnuson
B Steffensen
R J Klebe

First Published May 1, 1993

ARTICLE

Rapid characterization of HIV-1 sequence diversity using denaturing gradient gel electrophoresis and direct automated DNA sequencing of PCR products.

B Andersson
J H Ying
D E Lewis
R A Gibbs

First Published May 1, 1993

ARTICLE

Restriction-site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers.

G Sarkar
R T Turner
M E Bolander

First Published May 1, 1993

ARTICLE

Predominant mutations induced by the Thermococcus litoralis, vent DNA polymerase during DNA amplification in vitro.

P Keohavong
L Ling
C Dias
W G Thilly

First Published May 1, 1993

ARTICLE

PCR with degenerate primers containing deoxyinosine fails with Pfu DNA polymerase.

T Knittel
D Picard

First Published May 1, 1993

ARTICLE

Generation of cohesive ends on PCR products by UDG-mediated excision of dU, and application for cloning into restriction digest-linearized vectors.

C Smith
P J Day
M R Walker

First Published May 1, 1993

REVIEW

Quantitative PCR and RT-PCR in virology.

M Clementi
S Menzo
P Bagnarelli
A Manzin
A Valenza
P E Varaldo

First Published February 1, 1993

ARTICLE

PCR buffer optimization with uniform temperature regimen to facilitate automation.

M M Blanchard
P Taillon-Miller
P Nowotny
V Nowotny

First Published February 1, 1993

ARTICLE

Sensitive two-stage PCR of p53 genomic DNA exons 5-9.

W C Kusser
D B Levin
B W Glickman

First Published February 1, 1993

ARTICLE

Rapid preparation of limited biological samples for small-volume PCR.

B A Neilan
A Gurvitz
D A Leigh
L Y Lai
B McDonald

First Published February 1, 1993

ARTICLE

Efficient total gene synthesis of 1.35-kb hybrid alpha-lytic protease gene using the polymerase chain reaction.

S B Bambot
A J Russell

First Published February 1, 1993

ARTICLE

Synthesis and characterization of 5'-fluorescent-dye-labeled oligonucleotides.

W G Giusti
T Adriano

First Published February 1, 1993

ARTICLE

Differential detection of colinear genes and RNA transcripts by modified reverse transcription and PCR.

C Falcinelli
L J van Doorn
A van Belkum
W Quint

First Published February 1, 1993

ARTICLE

Genome walking with 2- to 4-kb steps using panhandle PCR.

D H Jones
S C Winistorfer

First Published February 1, 1993

ARTICLE

Simplified construction of a subtracted cDNA library using asymmetric PCR.

G Houge

First Published February 1, 1993