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ARTICLE

Quality assurance and use of PCR in clinical trials.

P S Reichelderfer
J B Jackson

First Published December 1, 1994

ARTICLE

A competitive deletion mutant quantitative PCR assay for angiotensin-converting enzyme mRNA in smooth muscle cells.

J J Lanzillo
X J Kong
B L Fanburg

First Published December 1, 1994

ARTICLE

Quantitative analysis of gene expression in different tissues by template-calibrated RT-PCR and laser-induced fluorescence.

W J Karges
R Gaedigk
H M Dosch

First Published December 1, 1994

ARTICLE

Rapid PCR site-directed mutagenesis.

M P Weiner
G L Costa

First Published December 1, 1994

ARTICLE

Alteration of hairpin ribozyme specificity utilizing PCR.

P DeGrandis
A Hampel
S Galasinski
J Borneman
A Siwkowski
M Altschuler

First Published December 1, 1994

ARTICLE

Quantitation of mRNA species by RT-PCR on total mRNA population.

S Hamoui
J P Benedetto
M Garret
J Bonnet

First Published December 1, 1994

ARTICLE

Single-strand conformational polymorphism.

K Fujita
J Silver

First Published December 1, 1994

ARTICLE

Direct gene quantitation by PCR reveals differential accumulation of ectopic enzyme in rat-1 cells, v-fos transformants, and revertants.

M B Bahramian
H Zarbl

First Published December 1, 1994

ARTICLE

An improved method for semiquantification of gene amplification from archival material.

M A Underwood
J M Bartlett
T G Cooke

First Published December 1, 1994

ARTICLE

In vitro synthesis of novel genes: mutagenesis and recombination by PCR.

A N Vallejo
R J Pogulis
L R Pease

First Published December 1, 1994

ARTICLE

Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products.

C Aslanidis
P J de Jong
G Schmitz

First Published December 1, 1994

ARTICLE

A comparison of methods for RNA extraction from lymphocytes for RT-PCR.

W Liedtke
L Battistini
C F Brosnan
C S Raine

First Published December 1, 1994

ARTICLE

Wax-embedded PCR reagents.

P Blair
R Ramanujam
B A Burdick

First Published December 1, 1994

ARTICLES

PCR analysis of the H ferritin multigene family reveals the existence of two classes of processed pseudogenes.

B Quaresima
M T Tiano
A Porcellini
P D'Agostino
M C Faniello
M A Bevilacqua
F Cimino
F Costanzo

First Published October 1, 1994

ARTICLES

Parameters affecting the sensitivities of dideoxy fingerprinting and SSCP.

Q Liu
S S Sommer

First Published October 1, 1994

ARTICLES

Amplification of RNA.

A Rashtchian

First Published October 1, 1994

ARTICLES

Assessment of colinearity between large cloned DNA fragments and genomic DNA.

P Verhasselt
G Volckaert

First Published October 1, 1994

ARTICLES

Detection of p53 gene mutation in cancer tissues by nonradioactive direct sequencing.

D Bautista
P Chaubert
J Bertoncini
J Benhattar

First Published October 1, 1994

ARTICLES

Rapid isolation of cDNA clones by aliquot testing via PCR amplification.

A Di Bacco
L Susani
A Villa
D Strina
A Frattini
P Vezzoni
I Zucchi

First Published October 1, 1994

ARTICLES

Detection of HCV RNA by the asymmetric gap ligase chain reaction.

R L Marshall
T G Laffler
M B Cerney
J C Sustachek
J D Kratochvil
R L Morgan

First Published October 1, 1994

ARTICLES

Amplification of gene fragments with very high G/C content: c7dGTP and the problem of visualizing the amplification products.

J Weiss
H D Zucht
W G Forssmann

First Published October 1, 1994

REVIEW

Expression-PCR (E-PCR): overview and applications.

D E Lanar
K C Kain

First Published October 1, 1994

ARTICLES

Reliability of PCR decontamination systems.

C Niederhauser
C Höfelein
B Wegmüller
J Lüthy
U Candrian

First Published October 1, 1994

ARTICLES

Analysis of nonspecific DNA synthesis during in situ PCR and solution-phase PCR.

G J Nuovo
P MacConnell
F Gallery

First Published October 1, 1994

ARTICLES

Effect of internal direct and inverted Alu repeat sequences on PCR.

W Ji
X Y Zhang
G S Warshamana
G Z Qu
M Ehrlich

First Published October 1, 1994

ARTICLES

Use of vectorette and subvectorette PCR to isolate transgene flanking DNA.

M J Allen
A Collick
A J Jeffreys

First Published October 1, 1994

ARTICLES

Detection and differential display of expressed genes by DDRT-PCR.

D Bauer
P Warthoe
M Rohde
M Strauss

First Published October 1, 1994

ARTICLES

Construction of phagemid display libraries with PCR-amplified immunoglobulin sequences.

H H Hogrefe
B Shopes

First Published October 1, 1994

ARTICLES

PCR-based method to map the bending locus of DNA molecules.

F Valle

First Published August 1, 1994

REVIEW

RNA fingerprinting by arbitrarily primed PCR.

M McClelland
J Welsh

First Published August 1, 1994

REVIEW

DNA fingerprinting by arbitrarily primed PCR.

M McClelland
J Welsh

First Published August 1, 1994

ARTICLES

A nonisotopic single-strand conformation polymorphism protocol using a direct blotting electrophoresis, a chemiluminescent detection system, and a multiplex approach.

H Knoblauch
N Weiss
I Eggersdorfer
C Keller
H Schuster

First Published August 1, 1994

ARTICLES

A DNA extraction method that allows reliable PCR amplification of MLO DNA from "difficult" plant host species.

K Gibb
A Padovan

First Published August 1, 1994

ARTICLES

Buffer components tailor DNA amplification with arbitrary primers.

G Caetano-Anollés
B J Bassam
P M Gresshoff

First Published August 1, 1994

ARTICLES

Computing genetic similarity coefficients from RAPD data: correcting for the effects of PCR artifacts caused by variation in experimental conditions.

W F Lamboy

First Published August 1, 1994

ARTICLES

Computing genetic similarity coefficients from RAPD data: the effects of PCR artifacts.

W F Lamboy

First Published August 1, 1994

ARTICLES

Determining relative microsatellite allele frequencies in pooled DNA samples.

H Khatib
A Darvasi
Y Plotski
M Soller

First Published August 1, 1994

REVIEW

Protocols for trapping internal and 3'-terminal exons.

P E Nisson
A Ally
P C Watkins

First Published August 1, 1994

ARTICLES

Development of a sensitive PCR to detect allele loss in a model hematopoietic neoplasm.

J Fairman
D Claxton
C L Williman
A B Deisseroth
L Nagarajan

First Published August 1, 1994

ARTICLES

A new diagnostic test for Gaucher disease suitable for population screening.

S A Sakallah
C Sansieri
D W Kopp
D L Cooper
J A Barranger

First Published August 1, 1994

ARTICLES

Comparison of gel matrices for resolving PCR-amplified DNA fingerprint profiles.

G He
C S Prakash
R L Jarret
S Tuzun
J Qiu

First Published August 1, 1994

REVIEW

Advanced methods in PCR product detection.

J G Lazar

First Published August 1, 1994

REVIEW

Strategies for direct sequencing of PCR-amplified DNA.

V B Rao

First Published August 1, 1994

REVIEW

On beyond classic RACE (rapid amplification of cDNA ends).

M A Frohman

First Published August 1, 1994

ARTICLES

A novel method to quantitate methylation of specific genomic regions.

M Heiskanen
A C Syvänen
H Siitari
S Laine
A Palotie

First Published August 1, 1994

ARTICLES

Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation.

K M Chumakov

First Published August 1, 1994

ARTICLES

PCR with end trimming and cassette ligation: a rapid method to clone exon-intron boundaries and a 5'-upstream sequence of genomic DNA based on a cDNA sequence.

H Iwahana
T Tsujisawa
R Katashima
K Yoshimoto
M Itakura

First Published August 1, 1994

ARTICLES

A simple PCR method for screening cDNA libraries.

D Alfandari
T Darribère

First Published August 1, 1994

ARTICLE

An internally controlled virion PCR for the measurement of HIV-1 RNA in plasma.

V Natarajan
R J Plishka
E W Scott
H C Lane
N P Salzman

First Published June 1, 1994

ARTICLE

Characterization of human AFLP systems apolipoprotein B, phenylalanine hydroxylase, and D1S80.

D Latorra
C M Stern
M S Schanfield

First Published June 1, 1994

ARTICLE

Improved quantitative PCR using nested primers.

L A Haff

First Published June 1, 1994

ARTICLE

Cloning and analysis of PCR-generated DNA fragments.

G L Costa
A Grafsky
M P Weiner

First Published June 1, 1994

ARTICLE

Rapid preparation of Thermus flavus DNA polymerase.

R A Harrell
R P Hart

First Published June 1, 1994

ARTICLE

Rapid RT-PCR amplification from limited cell numbers.

S Edmands
J Kirk
A Lee
J Radich

First Published June 1, 1994

ARTICLE

Inhibitory effect of salivary fluids on PCR: potency and removal.

A S Ochert
A W Boulter
W Birnbaum
N W Johnson
C G Teo

First Published June 1, 1994

ARTICLE

PCR mutagenesis and recombination in vivo.

D H Jones

First Published June 1, 1994

ARTICLE

Long-distance PCR.

O S Foord
E A Rose

First Published June 1, 1994

ARTICLE

Evaluation of bone marrow transplantation efficiency by competitive PCR on Y sequences.

S Patri
L Daheron
A Kitzis
J C Chomel

First Published June 1, 1994

ARTICLE

Sample preparation and PCR amplification from paraffin-embedded tissues.

C E Greer
C M Wheeler
M M Manos

First Published June 1, 1994

ARTICLE

Optimized conditions for cycle sequencing of PCR products.

J H Horton
M D Hagen
M S Ko

First Published June 1, 1994

ARTICLE

The use of the PCR to quantitate gene expression.

W C Gause
J Adamovicz

First Published June 1, 1994

ARTICLE

Mutagenic PCR.

R C Cadwell
G F Joyce

First Published June 1, 1994

ARTICLE

Specific immunoglobulin cDNA clones produced from hybridoma cell lines and murine spleen fragment cultures by 3SR amplification.

C A Stillman
P J Linton
P J Koutz
D J Decker
N R Klinman
T R Gingeras

First Published June 1, 1994

ARTICLE

Selective detection of hepatitis B virus RNA by PCR.

R Sallie

First Published June 1, 1994

ARTICLE

Quantitative PCR of bacteriophage lambda DNA using a second-generation thermocycler.

D M Kuehnelt
E Kukovetz
H P Hofer
R J Schaur

First Published June 1, 1994

ARTICLE

Quantitative analysis of CD34+ stem cells using RT-PCR on whole cells.

D A Molesh
J M Hall

First Published April 1, 1994

ARTICLE

Low-stringency PCR provides an internal control for negative results in PCR-based diagnosis.

O L de Caballero
E Dias Neto
M C Koury
A J Romanha
A J Simpson

First Published April 1, 1994

REVIEW

Issues of variability, carryover contamination, and detection in 3SR-based assays.

E Fahy
M Biery
M Goulden
S S Ghosh
T R Gingeras

First Published April 1, 1994

ARTICLE

The use of phosphorothioate primers and exonuclease hydrolysis for the preparation of single-stranded PCR products and their detection by solid-phase hybridization.

T T Nikiforov
R B Rendle
M L Kotewicz
Y H Rogers

First Published April 1, 1994

ARTICLE

A simplified method for PCR detection of hepatitis C virus RNA from human serum.

J Lai
A M Prince
L Wolfe
L Andrus

First Published April 1, 1994

REVIEW

Cycle sequencing.

K Kretz
W Callen
V Hedden

First Published April 1, 1994

ARTICLE

RNA associated with a heterodimeric protein that activates a meiotic homologous recombination hot spot: RL/RT/PCR strategy for cloning any unknown RNA or DNA.

W P Wahls

First Published April 1, 1994

ARTICLE

Mutation detection by fluorescent chemical cleavage: application to hemophilia B.

I I Haris
P M Green
D R Bentley
F Giannelli

First Published April 1, 1994

ARTICLE

Different methods of sample preparation influence sensitivity of Mycobacterium tuberculosis and Borrelia burgdorferi PCR.

W Liedtke
B Opalka
C W Zimmermann
E Schmid

First Published April 1, 1994

REVIEW

Overview of international PCR standardization efforts.

L E Bockstahler

First Published April 1, 1994

ARTICLE

Comparison of the enzyme-linked oligonucleotide sorbent assay to the 32P-labeled PCR/Southern blotting technique in quantitative analysis of human and rat mRNA.

J A Carcillo
A Parise
M Romkes-Sparks

First Published April 1, 1994

REVIEW

Basic methods for the detection of PCR products.

F J Jenkins

First Published April 1, 1994

ARTICLE

Optimized PCR using Vent polymerase.

K B Cease
C A Potcova
C J Lohff
M E Zeigler

First Published April 1, 1994

ARTICLE

Protocols for cloning and analysis of blunt-ended PCR-generated DNA fragments.

G L Costa
M P Weiner

First Published April 1, 1994

ARTICLE

Detection of DNA sequence variation via deliberate heteroduplex formation from genomic DNAs amplified en masse in "population tubes".

G Ruano
A S Deinard
S Tishkoff
K K Kidd

First Published February 1, 1994

ARTICLE

Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR.

C F Jin
M Mata
D J Fink

First Published February 1, 1994

ARTICLE

An efficient polymerase chain reaction approach for the quantitation of multiple RNAs in human tissue samples.

A Puntschart
K Jostarndt
H Hoppeler
R Billeter

First Published February 1, 1994

ARTICLE

Quantitation of specific transcripts by RT-PCR SNuPE assay.

J Singer-Sam

First Published February 1, 1994

ARTICLE

Amplification of ancient nuclear DNA from teeth and soft tissues.

S R Woodward
M J King
N M Chiu
M J Kuchar
C W Griggs

First Published February 1, 1994

ARTICLE

T-cassette ligation: a method for direct sequencing and cloning of PCR-amplified DNA fragments.

H Iwahana
K Yoshimoto
T Tsujisawa
M Itakura

First Published February 1, 1994

ARTICLE

Amplification of DNA from whole blood.

J Burckhardt

First Published February 1, 1994

REVIEW

Ligase chain reaction (LCR)--overview and applications.

M Wiedmann
W J Wilson
J Czajka
J Luo
F Barany
C A Batt

First Published February 1, 1994

REVIEW

Multiplex PCR: advantages, development, and applications.

M C Edwards
R A Gibbs

First Published February 1, 1994

ARTICLE

Secondary structure within PCR target sequences may facilitate heteroduplex production.

E Separovic
S A Nadin-Davis

First Published February 1, 1994

ARTICLE

A guide to the design and use of mismatched and degenerate primers.

S Kwok
S Y Chang
J J Sninsky
A Wang

First Published February 1, 1994

REVIEW

Isolating vector-insert junctions from yeast artificial chromosomes.

G A Silverman

First Published December 1, 1993

ARTICLE

PCR protocol for DNA recovery from Spurr's-embedded muscle biopsies.

F Capon
S L Cicero
G Novelli
B Dallapiccola

First Published December 1, 1993

ARTICLE

Evaluation of running conditions for SSCP analysis: application of SSCP for detection of point mutations in the LDL receptor gene.

T P Leren
K Solberg
O K Rødningen
L Ose
S Tonstad
K Berg

First Published December 1, 1993

ARTICLE

Effect of amplicon size on PCR detection of bacteria exposed to chlorine.

S C McCarty
R M Atlas

First Published December 1, 1993

ARTICLE

Protocol for ultraviolet irradiation of surfaces to reduce PCR contamination.

R W Cone
M R Fairfax

First Published December 1, 1993

ARTICLE

PCR-mediated detection of heteroplasmy in maize mitochondrial mutants.

J R Marienfeld
J Gu
K J Newton

First Published December 1, 1993

ARTICLE

General concepts for PCR primer design.

C W Dieffenbach
T M Lowe
G S Dveksler

First Published December 1, 1993

ARTICLE

Spermidine facilitates PCR amplification of target DNA.

C Y Wan
T A Wilkins

First Published December 1, 1993

ARTICLE

Quantitative PCR for hepatitis B virus with colorimetric detection.

P Lehtovaara
M Uusi-Oukari
P Buchert
M Laaksonen
M Bengtström
M Ranki

First Published December 1, 1993

ARTICLE

Effect of PCR conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions.

M A Jensen
N Straus

First Published December 1, 1993

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