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  1. .... Different from the gene-body localization of sense R-loops in Arabidopsis and Oryza sativa, maize sense R-loops mainly formed in both promoters and transcription termination sites (TTSs), and relatively less formed in gene bodies, indicating a species-specific R-loop pattern (Fig. 1E). The antisense R...
  2. ...for which total RNA can be extracted. To illustrate this, we profiled the starlet anemone Nematostella vectensis (metazoa), the fungus Neurospora crassa, rice plant leaves (Oryza sativa), and the protist Capsaspora owczarzaki (Sebé-Pedrós et al. 2016). These species were selected to broadly cover...
  3. ...; 39 Bio-Oriented Technology Research Advancement Institution, Minato-ku, Tokyo 105-0001, Japan. Abstract We present here the annotation of the complete genome of rice Oryza sativa L. ssp. japonica cultivar Nipponbare. All functional annotations for proteins and non-protein-coding RNA...
  4. ...that are targeted by the indicated small RNA. Figure 5. The genomic origins of the motif-targeting small RNAs, miR2118, and potential folding of transcripts containing these miRNAs. (A) The ;20-kb region that codes for most of the motif-targeting small RNAs. This region contains nine degenerate repeats each...
  5. ...sequencing sets. Duplicate sequences were tallied, then excised to form a nonredundant set of sequences. Sequences of <18 nt were removed. To find the origins of the small RNAs, BLAST was used to search the Oryza sativa sequence (TIGR Rice Annotation Release 5.0, http://www.tigr.org/tdb/e2k1/osa1/overview...
  6. ...Northern blot hybridization analysis using probes for specific MiS families to analyze the low-molecular-weight RNA fractions isolated from Nicotiana benthamiana , N. tabacum SR1, N. tabacum SR1: NN , N. glutinosa , S. demissum , S. tuberosum Kennebec, S. lycopersicum , Oryza sativa , and Arabidopsis...
  7. ...). Despite their inability to mobilize, degenerated TEs provide alternative DNA and RNA regulatory sequences (e.g., transcription factor binding sites, splicing information, transcriptional start or termination sites) or induce recombination owing to their repetitiveness (Ito et al. 2011; Kim and Zilberman...
  8. ...-confidence miRNAs using a computational pipeline, PIPmiR, specifically developed for the identification of plant miRNAs. PIPmiR uses a probabilistic model that combines RNA structure and expression information to identify miRNAs with high precision. Knockdown of three of the newly identified miRNAs results...
  9. ..., Oryza sativa, Sorghum bicolor, Setaria italica, and Zea mays. A single orthologous wheat gene was identified for 2686 (99.22%) of these, with 2665 (98.45%) Figure 2. Comparative alignment of TGACv1 scaffolds with the 3B BAC-based pseudomolecule. (A,C) Dot plots between TGACv1 scaffolds and 3B show...
  10. ...Department of Molecular Genetics, University Utrecht, NL-3584 CH Utrecht, The Netherlands Abstract Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization...
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