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  1. ...in Shigella spp. Appl. Environ. Microbiol. 57 : 2912 -2917. ↵ Jin, Q., Yuan, Z., Xu, J., Wang, Y., Shen, Y., Lu, W., Wang, J., Liu, H., Yang, J., Yang, F., et al. 2002 . Genome sequence of Shigella flexneri 2a: Insights into pathogenicity through comparison with s of Escherichia coli K12 and O157. Nucleic...
  2. ..., Blacksburg, Virginia 24061 USA Abstract Computer-based genome-wide screening of the DNA sequence of Escherichia coli strain K12 revealed tens of thousands of tandem simple sequence repeat (SSR) tracts, with motifs ranging from 1 to 6 nucleotides. SSRs were well distributed throughout the genome...
  3. ...and ligated into a pZero-based positive selection vector. The recombinant plasmids are electroporated into competent Escherichia coli cells to generate the GST library in preparation for DNA sequence analysis. View larger version: In this window In a new window Figure 1. Schematic for GST preparation...
  4. ...and evolution of s as the rate of nucleotide substitutions ( Graur et al. 1989 ; Zhang and Gerstein 2003c ) and the rate of DNA loss can be deduced from the study of these genomic “fossil records” ( Petrov et al. 1996 ; Petrov and Hartl 2000 ; Bensasson et al. 2001 ). Analysis of the multiple cytochrome c...
  5. ...and remove PCR inhibitors in previous studies on genera including Listeria (Skjerve et al. 1990), Mycobacterium (Grant et al. 1998), Escherichia coli O157:H7 (Fratamico et al. 1992), Actinobacillus (Angen et al. 2001), and also Chlamydia (Niesters et al. 1991; Hedrum et al. 1992). Using this combined...
  6. ...of Escherichia coli O157:H7 (accession no. NP_285744; Perna et al. 2001 ), whereas CcdBVfi shared only 35% identity with the toxin, CcdBO157 (accession no. NP_2857445.1). To functionally characterize this putative ccdAB system, the 739-bp Nsi I fragment from p669 encompassing the two ORFs of V. fischeri cassette...
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