Searching journal content for articles similar to Zee et al. 32 (11-12): 2092.

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  1. Method: Nanopore-based consensus sequencing enables accurate multimodal tumor cell-free DNA profiling
    • Li-Ting Chen,
    • Myrthe Jager,
    • Dàmi Rebergen,
    • Geertruid J. Brink,
    • Tom van den Ende,
    • Willem Vanderlinden,
    • Pauline Kolbeck,
    • Marc Pagès-Gallego,
    • Ymke van der Pol,
    • Nicolle Besselink,
    • Norbert Moldovan,
    • Nizar Hami,
    • Wigard P. Kloosterman,
    • Hanneke van Laarhoven,
    • Florent Mouliere,
    • Ronald Zweemer,
    • Jan Lipfert,
    • Sarah Derks,
    • Alessio Marcozzi,
    • and Jeroen de Ridder
    Genome Res. April 2025 35: 886-899; Published in Advance January 13, 2025, doi:10.1101/gr.279144.124
    ...with OVCA (Fig. 1E; Supplemental Table S1). NanoRCS libraries of cfDNA were sequenced on nanopore MinION and/or PromethION systems. To allow comparison with Illumina sequencing, shallow whole- sequencing (WGS) with NovaSeq was performed in parallel on all tumor cfDNA samples and three of the HC samples...
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  2. Method: Complete characterization of the human immune cell transcriptome using accurate full-length cDNA sequencing
    • Charles Cole,
    • Ashley Byrne,
    • Matthew Adams,
    • Roger Volden,
    • and Christopher Vollmers
    Genome Res. April 2020 30: 589-601; Published in Advance April 20, 2020, doi:10.1101/gr.257188.119
    ...). Next, we circularized the full-length cDNA and performed rolling circle amplification on the resulting circular DNA. This reaction generated long dsDNA containing multiple concatemeric copies of the original full-length cDNA. We either sequenced this DNA on the ONT MinION using the R2C2 protocol...
  3. Resource: Generation and analysis of a mouse multitissue genome annotation atlas
    • Matthew Adams and
    • Christopher Vollmers
    Genome Res. November 2024 34: 2108-2117; Published in Advance October 23, 2024, doi:10.1101/gr.279217.124
    ...to minimizing batch effects, pooling samples also streamlined sample preparation and sequencing. Further, because every sample was present in each sequencing library at approximately the same ratio we could sequence our sample pools across many ONT flow cells—both on the MinION and PromethION—and combine...
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