...resistance to MTX (Tarassov et al. 2008) in-frame, as well as out-of-frame as a control. Dhfr along with a HPH-resistance
module (on a pAG32-DHFR1,2-3; synthesized by Synbio Tech) were PCR amplified (Kapa HiFi DNA polymerase, Kapa Biosystems) using primers that, at each end, added homology regions flanking...