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Research: Nucleosome binding by TP53, TP63, and TP73 is determined by the composition, accessibility, and helical orientation of their binding sites
- Patrick D. Wilson,
- Xinyang Yu,
- Christopher R. Handelmann,
- and Michael J. Buck
Genome Res. March 2025 35: 404-416; Published in Advance February 10, 2025, doi:10.1101/gr.279541.124
..., and TP73To comprehensively examine the nucleosome-binding abilities of the TP53 family, we developed Pioneer-seq, which is an extension of low-throughput nucleosome-binding assays (Yu and Buck 2019). Pioneer-seq is a high-throughput competitive nucleosome-binding assay for gauging the binding affinities...
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Perspective: Unraveling the hidden complexity of cancer through long-read sequencing
- Qiuhui Li,
- Ayse G. Keskus,
- Justin Wagner,
- Michal B. Izydorczyk,
- Winston Timp,
- Fritz J. Sedlazeck,
- Alison P. Klein,
- Justin M. Zook,
- Mikhail Kolmogorov,
- and Michael C. Schatz
Genome Res. April 2025 35: 599-620; Published in Advance March 20, 2025, doi:10.1101/gr.280041.124
...of widespread mutations in TP53 (Nigro et al. 1989) and the molecular basis of the subtypes of breast cancers (Perou et al. 2000). While exome-wide studies were possible with these technologies, sample sizes were modest (Wood et al. 2007; Jones et al. 2009). Nevertheless, these technologies remain clinically...
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