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  1. ...; Supplemental Fig. S8). These findings are consistent with previous observations that BEAF-32, CP190, and Chromator are better predictors of TAD boundaries in Drosophila (Ramírez et al. 2018; Wang et al. 2018).View larger version: In this window In a new window Figure 3. Conservation of distinct classes of TAD...
  2. ...a function of the domain's position along the AP axis.Differential accessibility is a signature of axis patterning enhancersOpen chromatin regions in the Drosophila blastoderm correspond to a range of functional genomic elements, such as transcribed gene bodies, promoters, distal CREs, insulators...
  3. ...of these architectural proteins seems to disrupt TADs architecture unevenly, suggesting there are different types of borders (Zuin et al. 2014; Nora et al. 2017; Schwarzer et al. 2017). In contrast, TAD borders in Drosophila are occupied by a large set of insulator proteins, including CTCF, BEAF-32, Chromator (Chro), Cp...
  4. ...subclasses of a Drosophila insulator show distinct and cell type-specific genomic distributions. Genes Dev 23: 1338–1350. Butcher RD, Chodagam S, Basto R, Wakefield JG, Henderson DS, Raff JW, Whitfield WG. 2004. The Drosophila centrosome-associated protein CP190 is essential for viability but not for cell...
  5. ...Drosophila insulator proteins such as Suppressor of Hairy wing [SU(HW)] and Boundary Element Associated Factor of 32 kDa (BEAF-32) at the borders of H3K27me3 domains, which are also enriched for associated insulator proteins and additional cofactors. RNAi depletion of dCTCF and combinatorial knockdown...
  6. ...are organized into topologically associating domains (TADs), evolutionary conserved compact chromatin domains that influence gene expression. Mechanisms that underlie partitioning of the genome into TADs remain poorly understood. To explore principles of TAD folding in Drosophila melanogaster , we performed Hi...
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