Searching journal content for articles similar to Xiao et al. 14 (9): 1749.

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  1. ...accomplishes the conversion of luciferin to its oxi-derivative by the action of luciferase. The ensuing light output becomes proportional to the number of added bases, up to about four bases. To allow processivity of the method dNTP excess is degraded by apyrase, which is also present in the starting reaction...
  2. ...signal was obtained after 30 cycles with 1,000,000 specific counts, which is a 500-fold signal to background ratio. At 32 and 34 cycles, there was a slight but reproducible decrease in signal due to excess target molecules that compete for probe binding and decrease the AlphaScreen signal. To avoid...
  3. ...nucleotide extension assay was conducted with ThermoSequenase as described in Methods in the presence of 10 μ m ddNPTs. ( A ) Single-stranded target; ( B ) Target with 10% excess of complementary sequence; ( C ) Negative enzyme control. Primer Extension from BRCA1 PCR Product The primer extension assay...
  4. ...>4.3, and 913 had eQTL with LOD scores >7.0. Although some eQTL are false positives, these frequencies are far in excess of the numbers one would expect by chance alone. Approximately 34% (1171) of the mapped genes with eQTL exceeding 4.3 had a physical location coincident with the eQTL position...
  5. ...al. 1996a , b ; J. Bashkin, pers. comm.). DYEnamic ET primers and DYEnamic ET terminator kits with Thermo Sequenase DNA polymerse are optimized for use with MegaBACE1000. Read lengths in excess of 800 bp are possible with an average read length with dye primer chemistry of >550 bp at 98...
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