Searching journal content for articles similar to Wu et al. 21 (4): 578.

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  1. ...gamete cells to discern whether it either resembles or informs early pre-ZGA zebrafish embryo chromatin architecture. First, we assessed the higher-order architecture through advanced low-cell in situ Hi-C. The structure of zebrafish sperm, packaged by histones, lacks topological associated domains...
  2. ...of developmentally important genes. During spermatid maturation into sperm, these genes lose H3K4me2/3 and retain H3K27me3 marks. Experimental removal of these epigenetic marks at fertilization de-regulates gene expression in the resulting embryos in a paternal chromatin-dependent manner. This demonstrates...
  3. .... Genes for embryo development are packaged in blocks of multivalent chromatin in zebrafish sperm. Genome Res 21: 578–589. ↵Wu J, Huang B, Chen H, Yin Q, Liu Y, Xiang Y, Zhang B, Liu B, Wang Q, Xia W, et al. 2016. The landscape of accessible chromatin in mammalian preimplantation embryos. Nature 534: 652...
  4. ...-Cl at pH 8.0, 10 mM EDTA, 1% SDS). Protein was separated from Dynabeads by incubating for 15 min at 65°C on a shaking heat block at 1400 rpm. Eluates were de-crosslinked at 65°C overnight. Input chromatin (one-eighth of the total chromatin amount) was also de-crosslinked at this point. Following...
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