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ARTICLE: The use of phosphorothioate primers and exonuclease hydrolysis for the preparation of single-stranded PCR products and their detection by solid-phase hybridization.
- T T Nikiforov,
- R B Rendle,
- M L Kotewicz,
- and Y H Rogers
Genome Res. April 1994 3: 285-291
....J. Holloway, B., Erdman, D.D. Durigon, E.L. and Murtagh. J.J. 1993 . An exonuclease-amplification coupled capture technique improves detection of PCR product. Nucleic Acids Res. 21 : 3905 – 3906 . 19.. Gyllensten, U. , Erlich. H.A. Gyllensten, U. and Erlich. H.A. 1988 . Generation of single-stranded DNA...
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Method: Multiplexed massively parallel SELEX for characterization of human transcription factor binding specificities
- Arttu Jolma,
- Teemu Kivioja,
- Jarkko Toivonen,
- Lu Cheng,
- Gonghong Wei,
- Martin Enge,
- Mikko Taipale,
- Juan M. Vaquerizas,
- Jian Yan,
- Mikko J. Sillanpää,
- Martin Bonke,
- Kimmo Palin,
- Shaheynoor Talukder,
- Timothy R. Hughes,
- Nicholas M. Luscombe,
- Esko Ukkonen,
- and Jussi Taipale
Genome Res. June 2010 20: 861-873; Published in Advance April 8, 2010, doi:10.1101/gr.100552.109
...possible 14-bp sequences (from B) is incubatedwith a DNA-binding protein immobilized into awell of a 96-well plate, resulting in binding of DNA to the protein. After washing and elution, the resulting population of more specific sequences is amplified by PCR and subjected to high-throughput single...
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