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  1. ...function. Gene expression from bacterial inserts in BACs was detected ( Rondon et al. 1999 ; Xu et al. 1998 ) and a reduced maximum insert size was observed compared with nonprokaryotic inserts. The F-plasmid derived copy number control of the BAC vector allows one to two copies of BAC DNA per cell, which...
  2. ...was excluded to prevent the rupture of cellular DNA. Then, RNA was extracted immediately using the HiPure Liquid RNA Mini kit (Magen) at this BSL-2 laboratory. A NanoDrop One instrument was used to check RNA concentration and quality. Parallel treatment and analysis of plasma from healthy donors were used...
  3. ...omp A DNA of the Chlamydia spp. J. Clin. Microbiol. 30 : 1098 – 1104 . 10.. Wilton, S. , Cousins. D. Wilton, S. and Cousins. D. 1992 . Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. PCR Methods Applic. 1 : 269 – 273 . 11.. Way, J.S., , Josephson...
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