Searching journal content for articles similar to Weighardt et al. 3 (1): 77.

Displaying results 1-10 of 477
For checked items
  1. Method: Defining cell-type specificity at the transcriptional level in human disease
    • Wenjun Ju,
    • Casey S. Greene,
    • Felix Eichinger,
    • Viji Nair,
    • Jeffrey B. Hodgin,
    • Markus Bitzer,
    • Young-suk Lee,
    • Qian Zhu,
    • Masami Kehata,
    • Min Li,
    • Song Jiang,
    • Maria Pia Rastaldi,
    • Clemens D. Cohen,
    • Olga G. Troyanskaya,
    • and Matthias Kretzler
    Genome Res. November 2013 23: 1862-1873; Published in Advance August 15, 2013, doi:10.1101/gr.155697.113
    ...and validation. In silico nanodissection separated known podocyte genes from genes specific to the other glomerular cell lineages and tubular cells (Fig. 2), while a simple correlation-based approach failed to do so (Supplemental Fig. 1; Supplemental Methods). The applicability of our nanodissection strategy...
  2. Method: Application of microdroplet PCR for large-scale targeted bisulfite sequencing
    • H. Kiyomi Komori,
    • Sarah A. LaMere,
    • Ali Torkamani,
    • G. Traver Hart,
    • Steve Kotsopoulos,
    • Jason Warner,
    • Michael L. Samuels,
    • Jeff Olson,
    • Steven R. Head,
    • Phillip Ordoukhanian,
    • Pauline L. Lee,
    • Darren R. Link,
    • and Daniel R. Salomon
    Genome Res. October 2011 21: 1738-1745; Published in Advance July 14, 2011, doi:10.1101/gr.116863.110
    ...of interest by microdroplet PCR (Fig. 1A). To determine the appropriate design parameters for PCR of bisulfite-converted DNA, we first investigated the coverage attainable in silico for bisulfiteconverted genomic loci derived from the Sequenom Standard EpiPanel (Supplemental Table S1). Utilizing a simple...
  3. ARTICLE: Mutagenic PCR.
    • R C Cadwell and
    • G F Joyce
    Genome Res. June 1994 3: S136-S140
    ...Jolla, California 92037. U CA San Diego, La Jolla 6 1994 01 06 1994 3 3 3 6 ;3/6 6 3/6 S136 S140 Copyright © Cold Spring Harbor Laboratory Press References 1. Cha, R.S. and Thilly. W.G. 1993 . Specificity, efficiency, and fidelity of PCR. PCR Methods Applic. 3 : S18 – S29 . 2. Matteucci, M...
  4. ARTICLE: A simplified procedure for developing multiplex PCRs.
    • A P Shuber,
    • V J Grondin,
    • and K W Klinger
    Genome Res. December 1995 5: 488-493; doi:10.1101/gr.5.5.488
    ...parameters are identical for all UPS-tagged primers. We believe the enhanced specificity and efficiency that is conferred by the tagged primers is attributable to a nor- malization of the hybridization kinetics. During the early rounds of the PCR, molecules are synthesized that contain the tagged primers...
  5. REVIEW: The ligase chain reaction in a PCR world.
    • F Barany
    Genome Res. August 1991 1: 5-16; doi:10.1101/gr.1.1.5
    .... 1989 . In vivo footprinting of a muscle specific enhancer by ligation mediated PCR. Science 246 : 780 – 786 . 99. Pfeifer, G.P., , Tanquay, R.L. , Steigerwald, S.D. , Riggs. A.D. Pfeifer, G.P., Tanquay, R.L. Steigerwald, S.D. and Riggs. A.D. 1990 . In vivo footprint and methylation analysis by PCR...
  6. REVIEW: Expression-PCR (E-PCR): overview and applications.
    • D E Lanar and
    • K C Kain
    Genome Res. October 1994 4: S92-S96
    ...Conference on Parasitology, Abstract 546, p. 292; pers. comm. 13. Higuchi R., Drummel, B. and Saiki. R.K. 1988 . General method of in vitro preparation and specific mutagenesis of DNA fragments; study of protein DNA interactions. Nucleic Acids Res. 16 : 7351 – 7367 . M a n u a I III1|11 Expression-PCR (E-PCR...
  7. ARTICLE: Optimization and troubleshooting in PCR.
    • K H Roux
    Genome Res. April 1995 4: S185-S194
    ..., Oxford, England. 3.. Taguchi, G. 1986. Introduction to quality engineering. In Asian productivity organisation UNIPUB, New York, New York. 4.. Cobb, B.D. , Clarkson. J.M. Cobb, B.D. and Clarkson. J.M. 1994 . A simple procedure for optimizing the polymerase chain reaction (PCR) using modified Taguchi...
  8. ARTICLE: Optimized PCR using Vent polymerase.
    • K B Cease,
    • C A Potcova,
    • C J Lohff,
    • and M E Zeigler
    Genome Res. April 1994 3: 298-300
    ...in addition to quality was essential. Therefore, we sys- tematically examined a number of po- tentially important PCR conditions in a stepwise manner. This analysis led to a highly optimized procedure described below. MATERIALS AND METHODS Reactions were set up in 100-1~1 volumes in 0.5-ml polypropylene tubes...
  9. REVIEW: Sequencing of PCR-amplified DNA.
    • I S Bevan,
    • R Rapley,
    • and M R Walker
    Genome Res. May 1992 1: 222-228; doi:10.1101/gr.1.4.222
    .... S.S. Sarkar, G., Kapelner, S. and Sommer. S.S. 1990 . Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res. 18 : 7645 . 37. Hung, T., , Mak, K. , Fong. K. Hung, T., Mak, K. and Fong. K. 1990 . A specificity enhancer for polymerase chain reaction. Nucleic Acids Res. 18 : 4953...
  10. Methods: Multiplexed profiling of candidate genes for CpG island methylation status using a flexible PCR/LDR/Universal Array assay
    • Yu-Wei Cheng,
    • Carrie Shawber,
    • Dan Notterman,
    • Philip Paty,
    • and Francis Barany
    Genome Res. February 2006 16: 282-289; Published in Advance December 20, 2005, doi:10.1101/gr.4181406
    ...facilitated by gene-specific primers, initially during PCR and subsequently during LDR. Given the numerous duplications annotated and suspected in the human , this approach enhances the ability to reliably target the CpG islands in the locus of interest. Second, LDR allows accurate identification of low...
For checked items

Search Results

Next 10 » New Search

Modify Results

Citation format:
Results / page:
Results order:

This search

  • Alert me when new articles matching this search are published
  • Download all citations on this page to my citation manager

Preprint Server



Current Issue

  1. March 2026, 36 (3)
  1. Current Issue
  1. Alert me to new issues of Genome Research