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  1. .... 2021). BGCs were detected using antiSMASH version 7.1.0 (Blin et al. 2023). Plasmids and viruses/phages were identified using geNomad version 1.8.1 (Camargo et al. 2024), mob-suite version 3.1.9 (Robertson and Nash 2018), and VirSorter version 2.2.4 (Guo et al. 2021).The quality of the determined s...
  2. ...-mouse-NYU indicate the borders of contigs. Two similar flagellins (FliC3, and FliC4) in SFB-mouse-Japan are encoded at a position where only one copy is observed in the other s; sequence fragments from single cell SFB suggest the presence of two flagellins as well (this study). See Supplemental Figure S9...
  3. ...mapping split reads, which pinpoint breakpoint positions with nearly base-pair level accuracy. As a final filter, SMASH performs sequence homology Genome Research 301 www..org Structural variant discovery in NPC comparison of regions flanking the breakpoints using local alignment (Smith and Waterman 1981...
  4. ...in publicly available s, as found in the antiSMASH database (https://antismash-db.secondarymetabolites.org/#!/stats). Since NRPSs are very common (albeit elusive) in diverse bacterial data sets (Mukherjee et al. 2017) and since the downstream peptidogenomics analysis of NRPs is greatly impaired by fragmented...
  5. ...et al. 2018), or Halo- (Tovell et al. 2019) and SMASh-tags (Chung et al. 2015), allow acute and reversible depletion of TF proteins in response to a small molecule. These methods are particularly valuable because they bypass transcriptional adaptation and permit rapid TF loss, thereby minimizing...
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