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  1. ...in trans. View this table: In this window In a new window Table 2. Characterization of Nine Insertion Mutants of PVA Isolated from the Mutant cDNA Library PCR-based footprinting strategies have been used to map insertion sites efficiently in a relatively short region ( Smith et al. 1995 ; Singh et al. 1997...
  2. ...be performed should not be misinterpreted as evidence that the PCR is a simple process. In fact, quite the opposite is true. A single cycle of DNA polymerization is a complex pro- cess requiring the precise interaction of several components. The repetitive cycles characteristic of PCR provide an additional...
  3. ...are magnified (3×). Scale bar = 1 mm. Li et al. 1274 Genome Research www..org preparation was performed as described for cDNA library preparation (Miranda et al. 2013). We quantified individual gDNA libraries by quantitative PCR to determine the PCR cycle number for enriching and barcoding with indexing primers...
  4. ...noted (Tables 1 and 2 ). This is because the sequence for these subtelomeric regions is still under construction. PCR products spanning subtelomeric gaps are being sequenced currently. The optical map sizes were larger than those predicted from sequence for certain other fragments (Tables 1 and 2...
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