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Methods: A Strategy for Constructing Large Protein Interaction Maps Using the Yeast Two-Hybrid System:Regulated Expression Arrays and Two-Phase Mating
- Jinhui Zhong,
- Huamei Zhang,
- Clement A. Stanyon,
- Gerard Tromp,
- and Russell L. Finley, Jr.
Genome Res. December 2003 13: 2691-2699; Published in Advance January 1, 2003, doi:10.1101/gr.1134603
...that the ability to conduct large two-hybrid screens to map interactions on a -wide scale will continue to be an important part of functional genomics. The high-throughput two-hybrid strategy we described here should contribute to these efforts. METHODS Plasmids, Strains, and Yeast Two-Hybrid Assays The yeast two-hybrid...
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METHODS: Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs
- Manabu Nakayama,
- Reiko Kikuno,
- and Osamu Ohara
Genome Res. November 1, 2002 12: 1773-1784; Published in Advance October 15, 2002, doi:10.1101/gr.406902
...% of the noise caused by bait self-activation. Yeast Two-Hybrid Screening Using Bait and Prey Vectors With the Gateway System The Gateway system is a useful tool to easily transfer one or more cDNA fragments into various vectors, all the while maintaining orientation and reading frame in parallel reactions...

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