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METHODS: Parallel Construction of Orthologous Sequence-Ready Clone Contig Maps in Multiple Species
- James W. Thomas,
- Arjun B. Prasad,
- Tyrone J. Summers,
- Shih-Queen Lee-Lin,
- Valerie V.B. Maduro,
- Jacquelyn R. Idol,
- Joseph F. Ryan,
- Pamela J. Thomas,
- Jennifer C. McDowell,
- and Eric D. Green
Genome Res. August 1, 2002 12: 1277-1285; doi:10.1101/gr.283202
...varied from 56% in cow to 97% in chimpanzee (Fig. 2 C) and indicates that, while not uniform across all species, universal overgo hybridization probes are able to identify sufficiently redundant sets of clones for the assembly of sequence-ready BAC maps in multiple species. Construction of BAC Contig...
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INSIGHT/OUTLOOK: Sequence Ready—or Not?
- John D. McPherson
Genome Res. December 1, 1997 7: 1111-1113; doi:10.1101/gr.7.12.1111
...” similarities (see Fig. 2 ). It is a critical requirement for a sequence-ready map that the contigs have sufficient depth so that all restriction fragments can be validated by several overlapping clones. That is, if restriction fragment inconsistencies are observed, enough clones must be examined to establish...
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LETTER: A Sequence-Ready BAC Contig of the GABA_A Receptor Gene Cluster Gabrg1–Gabra2–Gabrb1 on Mouse Chromosome 5
- Andreas Lengeling,
- Tim Wiltshire,
- Chris Otmani,
- and Maja Bućan
Genome Res. August 1, 1999 9: 732-738; doi:10.1101/gr.9.8.732
...the construction of a sequence-ready contig of bacterial artificial chromosomes (BACs) encompassing this cluster. In the process of contig construction clones from 129/Sv and C57BL/6J BAC libraries were isolated. The assembled 1.3-Mb contig, consisting of 45 BACs, gives five- to sixfold coverage over the gene...
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RESOURCE: Construction of a BAC Contig Map of Chromosome 16q by Two-Dimensional Overgo Hybridization
- Cliff S. Han,
- Robert D. Sutherland,
- Phillip B. Jewett,
- Mary L. Campbell,
- Linda J. Meincke,
- Judy G. Tesmer,
- Mark O. Mundt,
- John J. Fawcett,
- Ung-Jin Kim,
- Larry L. Deaven,
- and Norman A. Doggett
Genome Res. May 1, 2000 10: 714-721; doi:10.1101/gr.10.5.714
...constructed with 6 of these &gt;3 Mb. These contigs cover &gt;85% of the long arm of chromosome 16. To confirm the overgo–BAC contigs and to select the minimal tiling set of BACs for high throughput sequencing, 3363 clones were fingerprinted by Eco RI digestion. One hundred six of these clones failed...
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LETTER: High-Resolution Landmark Framework for the Sequence-Ready Mapping of Xq23–q26.1
- Helen E. Steingruber,
- Andrew Dunham,
- Alison J. Coffey,
- Sheila M. Clegg,
- Gareth R. Howell,
- Gareth L. Maslen,
- Carol E. Scott,
- Rhian Gwilliam,
- Paul J. Hunt,
- Elizabeth C. Sotheran,
- Elizabeth J. Huckle,
- Sarah E. Hunt,
- Pawandeep Dhami,
- Cari Soderlund,
- Margaret A. Leversha,
- David R. Bentley,
- and Mark T. Ross
Genome Res. August 1, 1999 9: 751-762; doi:10.1101/gr.9.8.751
...∼15 Mb of DNA. The framework map is being used to assemble a sequence-ready map in large-insert bacterial clones, as part of an international effort to complete the sequence of the X chromosome. PAC and BAC contigs currently cover 18 Mb of the region, and from these, 12 Mb of finished sequence...
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LETTER: A 12-Mb Complete Coverage BAC Contig Map in Human Chromosome 16p13.1–p11.2
- Yicheng Cao,
- Hyung Lyun Kang,
- Xuequn Xu,
- Mei Wang,
- So Hee Dho,
- Jun Ryul Huh,
- Byeong-Jae Lee,
- Francis Kalush,
- Diana Bocskai,
- Yan Ding,
- Judith G. Tesmer,
- Jonghyeob Lee,
- Eunpyo Moon,
- Vesna Jurecic,
- Antonio Baldini,
- Heinz-Ulrich Weier,
- Norman A. Doggett,
- Melvin I. Simon,
- Mark D. Adams,
- and Ung-Jin Kim
Genome Res. August 1, 1999 9: 763-774; doi:10.1101/gr.9.8.763
....E. Lawrence Berkeley Laboratory, Berkeley, California 94720 USA Abstract We have constructed a complete coverage BAC contig map that spans a 12-Mb genomic segment in the human chromosome 16p13.1–p11.2 region. The map consists of 68 previously mapped STSs and 289 BAC clones, 51 of which...
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LETTER: A Contiguous 3-Mb Sequence-Ready Map in the S3–MX Region on 21q22.2 Based on High- Throughput Nonisotopic Library Screenings
- Thomas Hildmann,
- Xianging Kong,
- John O’Brien,
- Lisa Riesselman,
- Hoang-My Christensen,
- Emilie Dagand,
- Hans Lehrach,
- and Marie-Laure Yaspo
Genome Res. April 1, 1999 9: 360-372; doi:10.1101/gr.9.4.360
...on this filter; the arrow indicates clone 228A2. View larger version: In this window In a new window Figure 2. Contig and gene map between D21S3 and TMPRSS2 . The sequenced MTP consists of two BACs, 20 PACs, and five cosmids depicted in red. Sizes of clones are indicated in parentheses. Markers are shown above...
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METHODS: High-Resolution BAC-Based Map of the Central Portion of Mouse Chromosome 5
- Jonathan Crabtree,
- Tim Wiltshire,
- Brian Brunk,
- Shaying Zhao,
- Jonathan Schug,
- Christian J. Stoeckert, Jr.,
- and Maja Bucan
Genome Res. October 1, 2001 11: 1746-1757; doi:10.1101/gr.195101
...; Pennisi 2000 ; Marshall 2000 , 2001 ). Systematic restriction fragment analysis or “fingerprinting” and bacterial artificial chromosome (BAC)-end sequencing of all clones in the selected C57BL/6J (RPCI-23 and RPCI-24) BAC libraries will permit long-range association of sequence contigs. In the second...
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METHODS: Optical Mapping of BAC Clones from the Human Y Chromosome DAZ Locus
- Joseph Giacalone,
- Stephanie Delobette,
- Veronica Gibaja,
- Lei Ni,
- Yiannis Skiadas,
- Rong Qi,
- Joanne Edington,
- Zhongwu Lai,
- Damara Gebauer,
- Hongjuan Zhao,
- Thomas Anantharaman,
- Bhubaneswar Mishra,
- Laura G. Brown,
- Richa Saxena,
- David C. Page,
- and David C. Schwartz
Genome Res. September 1, 2000 10: 1421-1429; doi:10.1101/gr.112100
...us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs...
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RESOURCE: A BAC-Based Physical Map of the Chicken Genome
- Chengwei Ren,
- Mi-Kyung Lee,
- Bo Yan,
- Kejiao Ding,
- Bettye Cox,
- Michael N. Romanov,
- Jennifer A. Price,
- Jerry B. Dodgson,
- and Hong-Bin Zhang
Genome Res. December 2003 13: 2754-2758; doi:10.1101/gr.1499303
...In a new window Figure 1 Example of a chicken BAC contig anchored to the chromosome 1 genetic map of the chicken . This contig consists of 142 clones from three source BAC libraries, contains 903 unique fingerprint bands, and is estimated to span 4.01 Mb. The clones prefixed with “h” were from the Hin d...

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