Searching journal content for articles similar to Varley and Mitra 20 (9): 1279.

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  1. Review: Leveraging the power of long reads for targeted sequencing
    • Shruti V. Iyer,
    • Sara Goodwin,
    • and William Richard McCombie
    Genome Res. November 2024 34: 1701-1718; doi:10.1101/gr.279168.124
    ...of nontarget gDNA when coupled with Cas9 protection of ROI flanks, resulting in PCR-free enrichment. Unlike hybridization-based approaches, Cas9-based depletion does not exhibit sequence-specific bias, allowing for high-level multiplexing of targets. Additionally, since this strategy does not rely on DNA...
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  2. Method: Application of microdroplet PCR for large-scale targeted bisulfite sequencing
    • H. Kiyomi Komori,
    • Sarah A. LaMere,
    • Ali Torkamani,
    • G. Traver Hart,
    • Steve Kotsopoulos,
    • Jason Warner,
    • Michael L. Samuels,
    • Jeff Olson,
    • Steven R. Head,
    • Phillip Ordoukhanian,
    • Pauline L. Lee,
    • Darren R. Link,
    • and Daniel R. Salomon
    Genome Res. October 2011 21: 1738-1745; Published in Advance July 14, 2011, doi:10.1101/gr.116863.110
    ...–424. Tewhey R, Warner JB, Nakano M, Libby B, Medkova M, David PH, Kotsopoulos SK, Samuels ML, Hutchison JB, Larson JW, et al. 2009. Microdroplet-based PCR enrichment for large-scale targeted sequencing. Nat Biotechnol 27: 1025–1031. Varley KE, Mitra RD. 2010. Bisulfite Patch PCR enables multiplexed sequencing...
  3. Method: Multiplex mapping of chromatin accessibility and DNA methylation within targeted single molecules identifies epigenetic heterogeneity in neural stem cells and glioblastoma
    • Nancy H. Nabilsi,
    • Loic P. Deleyrolle,
    • Russell P. Darst,
    • Alberto Riva,
    • Brent A. Reynolds,
    • and Michael P. Kladde
    Genome Res. February 2014 24: 329-339; Published in Advance October 8, 2013, doi:10.1101/gr.161737.113
    ...the methylation status of all CG andGC sites (Pardo et al. 2011a). For studies requiring interrogation of multiple targets with greater than 203 coverage, MAPit-BGS would be laborious as well as material- and cost-prohibitive. Bisulfite patch PCR is a robust method for targeted nextgeneration bisulfite sequencing...
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