Searching journal content for articles similar to Varley and Mitra 18 (11): 1844.

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  1. Method: Bisulfite Patch PCR enables multiplexed sequencing of promoter methylation across cancer samples
    • Katherine Elena Varley and
    • Robi David Mitra
    Genome Res. September 2010 20: 1279-1287; Published in Advance July 13, 2010, doi:10.1101/gr.101212.109
    ...of samples. We sought to develop a method to perform highly multiplexed bisulfite sequencing across many patient samples simultaneously. We based our design on the Nested Patch PCR method that we developed for the multiplexed sequencing of loci to identify genetic mutations (Varley and Mitra 2008). Several...
  2. Methods: Direct multiplex sequencing (DMPS)—a novel method for targeted high-throughput sequencing of ancient and highly degraded DNA
    • Mathias Stiller,
    • Michael Knapp,
    • Udo Stenzel,
    • Michael Hofreiter,
    • and Matthias Meyer
    Genome Res. October 2009 19: 1843-1848; Published in Advance July 27, 2009, doi:10.1101/gr.095760.109
    .... Nested Patch PCR enables highly multiplexed mutation discovery in candidate genes. Genome Res 18: 1844–1850. Willerslev E, Cooper A. 2005. Ancient DNA. Proc Biol Sci 272: 3–16. Received May 6, 2009; accepted in revised form June 24, 2009. Stil ler et al . 1848 Genome Research www..org Direct multiplex...
  3. Review: Leveraging the power of long reads for targeted sequencing
    • Shruti V. Iyer,
    • Sara Goodwin,
    • and William Richard McCombie
    Genome Res. November 2024 34: 1701-1718; doi:10.1101/gr.279168.124
    ...-read target enrichment (Hodges et al. 2007; Turner et al. 2009; Mertes et al. 2011; Altmüller et al. 2014; Ballester et al. 2016) and typically rely on multiplexed PCR amplification (Jones et al. 2008; Tewhey et al. 2009) or oligonucleotide-based DNA hybridization capture (Albert et al. 2007; Hodges et al...
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  4. Research: Alternative polyadenylation drives oncogenic gene expression in pancreatic ductal adenocarcinoma
    • Swati Venkat,
    • Arwen A. Tisdale,
    • Johann R. Schwarz,
    • Abdulrahman A. Alahmari,
    • H. Carlo Maurer,
    • Kenneth P. Olive,
    • Kevin H. Eng,
    • and Michael E. Feigin
    Genome Res. March 2020 30: 347-360; Published in Advance February 6, 2020, doi:10.1101/gr.257550.119
    ...reveals APA as an underappreciated driver of protumorigenic gene expression in PDAC via the loss of miRNA regulation.Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with a 5-yr survival rate of 9% (Siegel et al. 2017). Extensive sequencing studies have uncovered recurrently mutated genes (KRAS...
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