Searching journal content for articles similar to Uberla et al. 1 (2): 136.

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  1. ARTICLE: Rapid construction of deleted DNA fragments for use as internal standards in competitive PCR.
    • C F Jin,
    • M Mata,
    • and D J Fink
    Genome Res. February 1994 3: 252-255
    .... and Blankenstein. T. 1991 . Generation of competitor DNA fragments for quantitative PCR. PCR Methods Applic. 1 : 136 – 139 . 3.. Stieger, M., , Demolliere, C. , Ahlborn-Laake, L. , Mous. J. Stieger, M., Demolliere, C. Ahlborn-Laake, L. and Mous. J. 1991 . Competitive polymerase chain reaction assay...
  2. ARTICLES: Real time quantitative PCR.
    • C A Heid,
    • J Stevens,
    • K J Livak,
    • and P M Williams
    Genome Res. October 1996 6: 986-994; doi:10.1101/gr.6.10.986
    ...the precursor of NF-kappa B p50 mRNA in mouse intestine: Quantitative analysis by competitive PCR. Biochim. Biophys. Acta 1215 : 157 – 162 . Thomas, P.S. 1980 . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. 77 : 5201 – 5205 . Williams, S., C. Schwer...
  3. REVIEW: Quantitative PCR and RT-PCR in virology.
    • M Clementi,
    • S Menzo,
    • P Bagnarelli,
    • A Manzin,
    • A Valenza,
    • and P E Varaldo
    Genome Res. February 1993 2: 191-196; doi:10.1101/gr.2.3.191
    ...to be used in the competitive RT-PCR (cRT- PCR) was obtained. Similarly, rapid methods to construct DNA competitors using either PCR and site-directed mu- tagenesis by overlap extension (37) or am- plification of two fragments whose sub- sequent ligation supplies an internally truncated competitor (43) have...
  4. ARTICLE: A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions.
    • R Ross,
    • R Kleiz,
    • and A B Reske-Kunz
    Genome Res. June 1995 4: 371-375
    ...the addition of equal amounts of competitor DNA fragments to the probes to be co- amplified during PCR reaction. (1-3) As the competitor fragments are added in equal amounts they should yield equal amounts of PCR products. If the frag- ments derived from mRNA and the com- petitor are distinguishable in size...
  5. ARTICLE: An evaluation of competitor type and size for use in the determination of mRNA by competitive PCR.
    • R K McCulloch,
    • C S Choong,
    • and D M Hurley
    Genome Res. February 1995 4: 219-226
    ...by usage of novel competitor DNA constructs in quantitative polymerase chain reaction. J. Exp. Med. 174 : 1259 – 1262 . 5. Siebert, P.D. and Larrick. J.W. 1993 . PCR mimics: Competitive DNA fragments for use as internal standards in quantitative PCR. BioTechniques 14 : 244 – 249 . 6. Fandrey, J. and Bunn...
  6. REVIEW: Quantitative or semi-quantitative PCR: reality versus myth.
    • F Ferre
    Genome Res. August 1992 2: 1-9; doi:10.1101/gr.2.1.1
    .... , Diamanstein, T. , Blankenstein. T. Uberla, K., Platzer, C. Diamanstein, T. and Blankenstein. T. 1991 . Generation of competitor DNA fragments for quantitative PCR. PCR Methods Applic. 1 : 136 – 139 . 33.. Zack, J.A., , Arrigo, S.J. , Weitsman, S.R. , Go, A.L. , Haislip, A. , Chen. I.S.Y. Zack, J.A., Arrigo, S...
  7. ARTICLE: Quantitative detection of reverse transcriptase-PCR products by means of a novel and sensitive DNA stain.
    • C Schneeberger,
    • P Speiser,
    • F Kury,
    • and R Zeillinger
    Genome Res. February 1995 4: 234-238
    ...(TA Cloning Sys- tem; Invitrogen, San Diego, CA) and FIGURE 4 Dilution series of a 219-bp PCR fragment, stained either with SYBR Green I (A) or ethidium bromide (B). 100 fg competitor RNA (-5• 10S/molecules) was reverse transcribed, Vso of the cDNA was used for PCR (30 cycles). 1 O.1 PCR product...
  8. ARTICLE: Accurate and absolute quantitative measurement of gene expression by single-tube RT-PCR and HPLC.
    • A Hayward-Lester,
    • P J Oefner,
    • S Sabatini,
    • and P A Doris
    Genome Res. December 1995 5: 494-499; doi:10.1101/gr.5.5.494
    ...with antisense competitors. BioTechniques 17 : 934 – 942 . 16.. Porcher, C., , Malinge, M.C. , Picat, C. , Grandchamp. B. Porcher, C., Malinge, M.C. Picat, C. and Grandchamp. B. 1992 . A simplified method for determination of specific DNA or RNA copy number using quantitative PCR and an automatic DNA sequencer...
  9. ARTICLE: Quantitative analysis of specific mRNA transcripts using a competitive PCR assay with electrochemiluminescent detection.
    • J A Heroux and
    • A M Szczepanik
    Genome Res. June 1995 4: 327-330
    ...templates. This assay offers the high sensitivity of ELCL detection of PCR product coupled with the ability to distinguish product mole- cules by way of a unique restriction site contained within the competitor tem- plate. These properties allow for the quantitative detection of low amounts of target DNA...
  10. ARTICLES: Quantification of cytokine mRNA expression by RT-PCR and electrochemiluminescence.
    • C Vandevyver,
    • K Motmans,
    • and J Raus
    Genome Res. September 1995 5: 195-201; doi:10.1101/gr.5.2.195
    ...for quan- titation of cDNA by PCR-based meth- ods, (1-4) most of them utilizing the prin- ciple of competitive PCR (C-PCR) in which a synthetic DNA fragment is co- amplified with the target cDNA seg- ment. We were interested in comparing the expression of cytokine genes in umer- ous samples of antigen...
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