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  1. ...sensitivity for identifying clone overlap ( Olson et al. 1986 ; Riles et al. 1993 ; Wong et al. 1997 ). In multiple-complete-digest restriction-fragment mapping, MCD, confirmed cosmids cloned from YACs used three independent digestions, and redundant clone coverage of from 15 to 30X was necessary for mapping...
  2. ...SNPs that correlate with disease loci ( Shi 2002 ). Nonetheless, restriction mapping has fundamental drawbacks that limit its utility for comparative genomics. Digestion of the DNA removes information regarding the ordering of the fragments, requiring the use of multiple enzymes to construct...
  3. ...a particular relative placement of two contigs. When the linking data contain errors, the general problem of globally satisfying all constraints is intractable ( Huson et al. 2001 ; Kent and Haussler 2001 ). Interestingly, the associated contig orientation problem is also intractable, as is the complementary...
  4. ....G. , White O. , Adams M.D. , Kerlavage A.R. ( 1995 ) TIGR assembler: A new tool for assembling large shotgun sequencing projects. Genome Sci. Tech. 1 : 9 – 19 . ↵ Thayer E.C. , Olson M.V. , Karp R.M. ( 1999 ) Error checking and graphical representation of multiple-complete-digest (MCD) restriction-fragment...
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