Searching journal content for articles similar to Temple et al. 19 (12): 2324.

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  1. ...The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) The MGC Project Team 1 , 2 Abstract The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly...
  2. ...-throughput (retro) transfection and image acquisition High-throughput transfections of the expression-ready subset of the Mammalian Gene Collection were performed essentially as described ( Chanda et al. 2003 ; Huang et al. 2004 ; http://function.gnf.org ). This library, consisting of 7364 unique full...
  3. ...requires additional support such as multiple EST matches or similarity to genes in another species before pure predictions rise to the status of confirmed genes. Thus, the sequencing of full-length cDNAs by the Mammalian Gene Collection (MGC) and the RIKEN group (Carninci et al. 2002 , 2003 ; Waterston et...
  4. .... Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl. Acad. Sci. 99 : 16899 -16903. ↵ The MGC Project Team. 2004 . The status, quality and expansion of the NIH full-length cDNA project: The mammalian gene collection (MGC). Genome Res. 14 : 2121 -2127...
  5. ...regulatory elements, such as enhancers. Strategies that employ retroviral promoter traps have been used in mammalian cell lines and transgenic mice to isolate a few promoters, to identify developmental genes, and to study differentially regulated genes ( Von Melchner et al. 1990 ; Friedrich and Soriano 1991...
  6. ...the three databases of BodyMap, the Mammalian Gene Collection (MGC), and the National Eye Institute NEIBank. First, we created a single set of genes from the three distinct databases of gene expression for the human eye (see Methods section). Next, we obtained full-length gene sequences by comparing...
  7. ...brain Marathon-Ready cDNAs. A cDNA sequence from GenBank, BC005996 (Mammalian gene collection, http://mgc.nci.nih.gov ) and an EST contiguous sequence assembled from EST sequences AL540640 , AL545680 , and AL571299 match the 9pter sequence exactly and are therefore not shown. Blue residues are identical...
  8. ...that are as complete and accurate as possible, by some reasonable working definition of the gene. Also needed are essential reagents related to genes, such as cDNA clones. The Mammalian Gene Collection (MGC) project was created to provide the community with a representative high-quality full-length cDNA clone...
  9. ...isolation eSTs must be mapped in an expensive "top down" ap- proach using -wide reagents (Cox et al. 1990; Green and Olson 1990; Khan et al. 1991; Wilcox et al. 1991; Cohen et al. 1993; Polymero- poulos et al. 1993). Both approaches result in gene maps (and eST data bases) that will be in- complete if only...
  10. ...genetic method for constructing high-resolution maps of mammalian chromosomes. Science 250 : 245 – 250 . Fields, C., , Adams, M. , White, O. , Venter. J.C. Fields, C., Adams, M. White, O. and Venter. J.C. 1994 . How many genes in the human ? Nature Genet. 7 : 345 – 346 . Gardiner, K. , Mural. R. Gardiner...
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