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  1. ...transcriptomic, genomic, and histone modification data for Anolis and representative tetrapod species (Fig. 1; Methods). For Anolis, we collected transcriptome data using high-throughput strand-specific RNA sequencing (RNA-seq) for 30 adult and 48 embryonic samples (covering most of prehatching development...
  2. ...selected embryos within the pharyngula period for these two organisms (Irie andKuratani 2011). For Xenopus, previously published data from stage 24–26 embryos were included in our study (Tan et al. 2013). For mouse, we performed a complete RNA sequencing analysis in duplicate using 10.5-d embryos (Pearson...
  3. ...- assembly was performed with the newly developed assembler PLATANUS, which is optimized for short-read data from high-throughput sequencers. See Supplemental Information 2.3 for details. Genome browser The Coelacanth Genome Browser has been established using the assembled sequence and RNA-seq data...
  4. ...on different chromosomes using published RNA sequencing (RNA-seq) data (Supplemental Fig. S13; Supplemental Table S14). In C. mucedo, both the Hox4b and Lox5b genes separated from the main cluster are expressed more highly than their main cluster counterparts Hox4a and Lox5a (paired t-tests P = 0.0009 and P...
  5. ...structure based on known lineage groups (cluster purity = 0.812, where cluster purity = 1.0 − [misaligned dendrites/total dendrites]) (Fig. 2C). A previous study has reported that NJT built using ±1.5 kb H3K4me1 densities of long-intergenic noncoding RNAs (lincRNAs) TSSs is the best lineage classification...
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