Searching journal content for articles similar to Tallack et al. 20 (8): 1052.

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  1. ...of global gene regulation by KLF1 using traditional oligonucleotide microarray technology and, more recently, ChIP-seq technology have provided valuable insights into the KLF1-dependent erythroid transcriptome (Drissen et al. 2005; Hodge et al. 2006; Tallack et al. 2010; Pilon et al. 2011). These studies...
  2. ...proFigure 1. Comparison of ChIP-seq data for transcription factor occupancy between primary erythroid cells and the G1E cell system. (A) Factor binding and histone modification profiles are shown for the Hba locus encoding alpha-globins (left) and the Hbb locus encoding beta-globins (right...
  3. ...network We set out to identify targets of TAL1 with functions in erythropoiesis that may contribute to the erythroid phenotype of the Tal1RER/RERmousewhen their expression is perturbed. From the list of genes identified through ChIP-seq, 80 loci were selected that (1) contained sequences bound by wild...
  4. ..., at multiple stages of hematopoietic differentiation. We combined ChIP-seq and RNA-seq data in six mouse cell types representing a progression from multilineage precursors to differentiated erythroblasts and megakaryocytes. We found that sites of occupancy shift dramatically during commitment to the erythroid...
  5. ...genome;20/12/1748 1088-9051 Erratum Erratum Genome Research 20: 1052–1063 (2010) A global role for KLF1 in erythropoiesis revealed by ChIP-seq in primary erythroid cells Michael R. Tallack, Tom Whitington, Wai Shan Yuen, Elanor N. Wainwright, Janelle R. Keys, Brooke B. Gardiner, Ehsan Nourbakhsh...
  6. ...differentiate into a megakaryocyte-like state, they lose their potential to differentiate into erythroid cells. We confirmed this with qPCR for KLF1, an erythroid marker, which decreases in expression over differentiation (Supplemental Fig. S1B; Kuvardina et al. 2015). We then performed in situ Hi-C on four...
  7. ...polymorphisms are frequently prioritized by intersection with annotated regulatory elements or their potential to alter derived TF binding motifs. Major drawbacks of such approaches are their dependency on incomplete ChIP-seq data and associated transcription factor position weight matrices (PWMs) in specific...
  8. ...to a more complex lineage structure and for -wide sequencing data, we used ChIP-seq data from a comprehensive study of hematopoiesis (Fig. 1C) by Lara-Astiaso et al. (2014) that measured four chromatin marks in 16 different cell types using ChIP-seq. CMINT outperforms other approaches to finding modules...
  9. ...indicated by arrows and exons represented by black rectangles. ChIP-seq data showing transcription factor occupancy and histonemarks in ERY, MEG, and HPC-7 cells are indicated below (Wilson et al. 2010). A GATA1 OS (boxed) in the Bcl2l1 gene (C ) overlaps with a KLF1 OS identified previously in murine fetal...
  10. ...randomized ChIP-seq data sets were used (data not shown). To gain further insight into the features of promoter-interacting regions, we defined a set of chromatin states in ESCs, characterized by distinct combinations of factor occupancy and histone modifications (Supplemental Fig. 3H), and analyzed...
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