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Method: Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA
- Romualdas Vaisvila,
- V.K. Chaithanya Ponnaluri,
- Zhiyi Sun,
- Bradley W. Langhorst,
- Lana Saleh,
- Shengxi Guan,
- Nan Dai,
- Matthew A. Campbell,
- Brittany S. Sexton,
- Katherine Marks,
- Mala Samaranayake,
- James C. Samuelson,
- Heidi E. Church,
- Esta Tamanaha,
- Ivan R. Corrêa, Jr.,
- Sriharsa Pradhan,
- Eileen T. Dimalanta,
- Thomas C. Evans, Jr.,
- Louise Williams,
- and Theodore B. Davis
Genome Res. July 2021 31: 1280-1289; Published in Advance June 17, 2021, doi:10.1101/gr.266551.120
..., they are represented as thymines, whereas 5hmCs are sequenced as cytosines. A comparison of ACE-seq libraries to an unconverted identifies 5hmCs to single-base resolution (Schutsky et al. 2018). Recently, TET-assisted pyridine borane sequencing (TAPS) was described, and it combines an enzymatic step followed...
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Mini-Review: Evolution of genome-wide methylation profiling technologies
- Carolina Montano and
- Winston Timp
Genome Res. April 2025 35: 572-582; doi:10.1101/gr.278407.123
...uniform and less biased GC coverage, and even the ability to differentiate between 5mC and 5-hydroxymethylcytosine (5hmC) in some cases.Single moleculeDirect DNA sequencing using long-read sequencing (LRS) platforms like Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio) offers new...
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