Searching journal content for articles similar to Sun et al. 22 (7): 1350.

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  1. Method: Genome-wide dynamics of RNA synthesis, processing, and degradation without RNA metabolic labeling
    • Mattia Furlan,
    • Eugenia Galeota,
    • Nunzio Del Gaudio,
    • Erik Dassi,
    • Michele Caselle,
    • Stefano de Pretis,
    • and Mattia Pelizzola
    Genome Res. October 2020 30: 1492-1507; Published in Advance September 25, 2020, doi:10.1101/gr.260984.120
    ...be in silico separated from pre-existing RNA (Herzog et al. 2017; Baptista and Dölken 2018; Jürges et al. 2018; Schofield et al. 2018). A number of methods were developed for the quantification of RNA dynamics via metabolic labeling, including INSPEcT (de Pretis et al. 2015), DRUID (Lugowski et al. 2018), cDTA...
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  2. Research: Heritable variation of mRNA decay rates in yeast
    • Jennifer M. Andrie,
    • Jon Wakefield,
    • and Joshua M. Akey
    Genome Res. December 2014 24: 2000-2010; Published in Advance September 25, 2014, doi:10.1101/gr.175802.114
    ...-regulatory differences accumulate at a faster rate than transregulatory differences between closely related species (Wittkopp et al. 2008; Tirosh et al. 2009; Romero et al. 2012). The balance between mRNA synthesis and decay determines steady-state levels of transcript abundance, and genetic variation affecting either...
  3. Research: Global analyses of the dynamics of mammalian microRNA metabolism
    • Elena R. Kingston and
    • David P. Bartel
    Genome Res. November 2019 29: 1777-1790; Published in Advance September 13, 2019, doi:10.1101/gr.251421.119
    ..., these results establish a framework for describing the dynamics and regulation of miRNAs throughout their life cycle.MicroRNAs (miRNAs) are small RNAs that mediate posttranscriptional gene regulation by targeting mRNAs for degradation and translational repression. In animals, miRNAs typically recognize their mRNA...
  4. Method: Capturing in vivo RNA transcriptional dynamics from the malaria parasite Plasmodium falciparum
    • Heather J. Painter,
    • Manuela Carrasquilla,
    • and Manuel Llinás
    Genome Res. June 2017 27: 1074-1086; Published in Advance April 17, 2017, doi:10.1101/gr.217356.116
    ...transcriptomic approaches such as DNA microarrays or RNA-seq. This mRNA capture method from FCUexpressing parasites can be used to temporally profile the transcriptional dynamics of the developing Plasmodium parasite at all stages of development. Results Engineering a functional pyrimidine salvage pathway...
  5. Research: Divergence of a conserved elongation factor and transcription regulation in budding and fission yeast
    • Gregory T. Booth,
    • Isabel X. Wang,
    • Vivian G. Cheung,
    • and John T. Lis
    Genome Res. June 2016 26: 799-811; Published in Advance May 12, 2016, doi:10.1101/gr.204578.116
    .... Similar to an approach developed previously for microarray analysis of labeled mRNA (Sun et al. 2012), we included an exogenous, whole yeast spike-in control in our experiments, which was carried out as follows: All samples and biological replicates to be compared were required to have precisely the same...
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